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Scientific article
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English

Fatty acids do not activate UCP2 in pancreatic beta cells: comparison with UCP1

Contributors

Galetti, Sandrine; Sarre, Alexandre; Perreten, Hélène; Produit-Zengaffinen, Nathalie; Muzzin, Patrick; Assimacopoulos, Françoise

Published in

Pflügers Archiv, vol. 457, no. 4, p. 931-940

Collection

  • Open Access - Licence nationale Springer

Publication date

2009

Abstract

UCP2 is expressed in pancreatic beta cells where its postulated uncoupling activity will modulate glucose-induced changes in ATP/ADP ratio and insulin secretion. The consequences of UCP2 over/underexpression on beta-cell function has mainly been studied in the basal state; however, a UCP has no uncoupling activity unless stimulated by fatty acids and/or reactive oxygen species. Here, UCP2 was overexpressed in INS-1 cells and parameters reflecting mitochondrial coupling measured in the basal state and after stimulation by fatty acids. For comparison, UCP1 was expressed to similar levels and the same parameters measured. Neither UCP1 expression nor UCP2 overexpression modified basal or glucose-stimulated metabolic changes. Upon addition of fatty acids, UCP1-expressing cells displayed the expected mitochondrial uncoupling effect, while UCP2 did not elicit any measurable change in mitochondrial function. Taken together, our data demonstrate that, in pancreatic beta-cells, UCP2 has no uncoupling activity in the basal state or after fatty acid stimulation.

Keywords

  • Adenosine Diphosphate/metabolism
  • Adenosine Triphosphate/metabolism
  • Animals
  • Cell Line
  • Fatty Acids/metabolism
  • Humans
  • Insulin/metabolism
  • Insulin-Secreting Cells/cytology/metabolism
  • Ion Channels
  • Membrane Potential, Mitochondrial/physiology
  • Mitochondria/metabolism
  • Mitochondrial Proteins
  • Oxygen Consumption
  • Rats
  • Uncoupling Agents/metabolism

UNIGE affiliation entities

Citation (ISO format)

GALETTI, Sandrine et al. Fatty acids do not activate UCP2 in pancreatic beta cells: comparison with UCP1. In: Pflügers Archiv, 2009, vol. 457, n° 4, p. 931–940. doi: 10.1007/s00424-008-0548-8

Main files (1)

Article (Published version)

Identifiers

Additional URL for this publication

http://www.springerlink.com/content/h00617331235t242/fulltext.html

Journal ISSN

0031-6768
692views
393downloads

Technical informations

Creation

20/05/2009 16:24:00

First validation

20/05/2009 16:24:00

Update

14/03/2023 15:05:22

Status update

14/03/2023 15:05:22

Last indexation

29/10/2024 11:35:02
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