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Fatty acids do not activate UCP2 in pancreatic beta cells: comparison with UCP1

Galetti, Sandrine
Sarre, Alexandre
Perreten, Hélène
Published in Pflügers Archiv. 2009, vol. 457, no. 4, p. 931-40
Collection Open Access - Licence nationale Springer
Abstract UCP2 is expressed in pancreatic beta cells where its postulated uncoupling activity will modulate glucose-induced changes in ATP/ADP ratio and insulin secretion. The consequences of UCP2 over/underexpression on beta-cell function has mainly been studied in the basal state; however, a UCP has no uncoupling activity unless stimulated by fatty acids and/or reactive oxygen species. Here, UCP2 was overexpressed in INS-1 cells and parameters reflecting mitochondrial coupling measured in the basal state and after stimulation by fatty acids. For comparison, UCP1 was expressed to similar levels and the same parameters measured. Neither UCP1 expression nor UCP2 overexpression modified basal or glucose-stimulated metabolic changes. Upon addition of fatty acids, UCP1-expressing cells displayed the expected mitochondrial uncoupling effect, while UCP2 did not elicit any measurable change in mitochondrial function. Taken together, our data demonstrate that, in pancreatic beta-cells, UCP2 has no uncoupling activity in the basal state or after fatty acid stimulation.
Keywords Adenosine Diphosphate/metabolismAdenosine Triphosphate/metabolismAnimalsCell LineFatty Acids/metabolismHumansInsulin/metabolismInsulin-Secreting Cells/cytology/metabolismIon ChannelsMembrane Potential, Mitochondrial/physiologyMitochondria/metabolismMitochondrial ProteinsOxygen ConsumptionRatsUncoupling Agents/metabolism
PMID: 18626658
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GALETTI, Sandrine et al. Fatty acids do not activate UCP2 in pancreatic beta cells: comparison with UCP1. In: Pflügers Archiv, 2009, vol. 457, n° 4, p. 931-40. https://archive-ouverte.unige.ch/unige:1682

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Deposited on : 2009-05-20

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