Scientific article
English

Kinetics of blood component adsorption on poly(D,L-lactic acid) nanoparticles: evidence of complement C3 component involvement

Published inJournal of biomedical materials research, vol. 37, no. 2, p. 229-234
Publication date1997
Abstract

After intravenous administration, nanoparticles suffer a major drawback in that they are rapidly and massively taken up by the cells of the mononuclear phagocyte system. The mechanisms involved in the opsonization, adhesion, and internalization of biodegradable nanoparticles by the mononuclear phagocyte system are still poorly understood. In this work, the kinetics of blood protein absorption onto nanoparticles of poly (D,L-lactic acid) prepared by the salting-out technique was investigated. Nanoparticles of 312 nm were incubated for variable periods of time (5-60min) in human serum and citrated plasma. After incubation, the particules were washed and the proteins detached from them, denatured, and analyzed by two-dimensional polyacrylamide gel electrophoresis. In plasma, the predominant protein was immunoglobulin G (IgG), and the amount adsorbed was not dependent on incubation time. Albumin amounts were high for short incubation periods but decreased as a function of time, whereas apolipoprotein E levels increased significantly as a function of the incubation period. Owing to the possible complement cascade inactivation by addition of citrate to plasma, the kinetics of absorption was also evaluated in serum. In this medium, absorption of complement C3 components onto the surface of the nanoparticles was clearly evidenced by spots of increasing intensity and area, reaching levels comparable to those of the omnipresent IgG. This result confirms the important role of complement components in the opsonization process of poly (D,L-lactic acid) particles.

Keywords
  • Poly(lactic acid)
  • Nanoparticles
  • Proetin absorption
  • Two-dimensional polyacrylamide gel electrophoresis
  • Complement activation
Citation (ISO format)
ALLÉMANN, Eric et al. Kinetics of blood component adsorption on poly(D,L-lactic acid) nanoparticles: evidence of complement C3 component involvement. In: Journal of biomedical materials research, 1997, vol. 37, n° 2, p. 229–234. doi: 10.1002/(SICI)1097-4636(199711)37:2<229::AID-JBM12>3.0.CO;2-9
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ISSN of the journal0021-9304
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