en
Book chapter
English

Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa

Published inc-di-GMP Signaling : methods and protocols, Editors Sauer, K., p. 213-224
PublisherNew York : Humana press
Collection
  • Methods in molecular biology; 1657
Publication date2017
Abstract

Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems.

eng
Keywords
  • Bacterial toxins
  • Cyclic di-GMP
  • Galleria mellonella
  • Pseudomonas aeruginosa
  • Type III secretion
  • Type VI secretion
  • Animals
  • Bacterial Toxins / metabolism
  • Cyclic GMP / analogs & derivatives
  • Cyclic GMP / metabolism
  • Moths / microbiology
  • Mutation
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / metabolism
  • Pseudomonas aeruginosa / pathogenicity
  • Type III Secretion Systems / genetics
  • Type III Secretion Systems / metabolism
  • Type VI Secretion Systems / genetics
  • Type VI Secretion Systems / metabolism
Affiliation Not a UNIGE publication
Citation (ISO format)
MCCARTHY, Ronan R, VALENTINI, Martina, FILLOUX, Alain. Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa. In: c-di-GMP Signaling : methods and protocols. New York : Humana press, 2017. p. 213–224. (Methods in molecular biology) doi: 10.1007/978-1-4939-7240-1_17
Main files (1)
Book chapter (Published version)
accessLevelRestricted
Identifiers
ISBN978-1-4939-7239-5
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Creation10/10/2022 3:08:00 PM
First validation10/10/2022 3:08:00 PM
Update time03/16/2023 10:49:22 AM
Status update03/16/2023 10:49:21 AM
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