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Functional properties of the neuronal nicotinic acetylcholine receptor β3 promoter in the developing central nervous system

Published inThe Journal of biological chemistry, vol. 273, no. 24, p. 15131-15137
Publication date1998
Abstract

Within the chick central nervous system, expression of the β3nicotinic acetylcholine receptor gene is restricted to a subset of retinal neurons, the majority of which are ganglion cells. Transient transfection in retinal neurons and in neural and non-neural cells from other regions of the click embryo allowed the indentification of the cis-regulatory domain of the β3 gene. Within this domain, a 75-base pair fragment located immediately upstream of the transcription start site suffices to reproduce the neuron-specific expression pattern of β3. This fragment encompasses an E-box and a CAAT box, both of which are shown to be key positive regulatory elements of the β3 promoter. Co-transfection experiments into retinal, telencephalic, and tectal neurons with plasmid reporters of β3 promoter activity and a number of vectors expressing different neuronal (ASH-1, NeuroM, NeuroD, CTF-4) and non-neuronal (MyoD) basic helix-loop-helix transcription factors indicate that the cis-regulatory domain of β3 has the remarkable property of discriminating accurately between related members of the basic helix-loop-helix protein family. The sequence located immediately 3' of the E-box participates in this selection, and the E-box acts in concert with the nearby CAAT box.

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Citation (ISO format)
ROZTOCIL, Tomas et al. Functional properties of the neuronal nicotinic acetylcholine receptor <i>β</i>3 promoter in the developing central nervous system. In: The Journal of biological chemistry, 1998, vol. 273, n° 24, p. 15131–15137. doi: 10.1074/jbc.273.24.15131
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ISSN of the journal0021-9258
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