We report the presence of habitual ground nesting in a newly studied East African chimpanzee (Pan troglodytes schweinfurthii) population in the Bugoma Central Forest Reserve, Uganda. Across a 2-year period we encountered 989 night-nests, 205 of which were classified as ground nests, a rate of ~21%. We find no preliminary evidence of socio-ecological factors that would promote its use and highlight local factors that appear to make its use disadvantageous. While further study is required to establish this behaviour as meeting the strict criteria for non-human animal culture, we support the argument that the wider use of population and group-specific behavioural repertoires in flagship species, such as chimpanzees, offers a tool to promote the urgent conservation action needed to protect threatened ecosystems, including the Bugoma forest.