Scientific article
Open access

Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering

Published inBiomedicines, vol. 10, no. 11, 2817
Publication date2022-11-04
First online date2022-11-04

Background: In end-stage chronic liver disease, transplantation represents the only curative option. However, the shortage of donors results in the death of many patients. To overcome this gap, it is mandatory to develop new therapeutic options. In the present study, we decellularised pig livers and reseeded them with allogeneic porcine mesenchymal stromal cells (pMSCs) to understand whether extracellular matrix (ECM) can influence and/or promote differentiation into hepatocyte-like cells (HLCs).

Methods: After decellularisation with SDS, the integrity of ECM-scaffolds was examined by histological staining, immunofluorescence and scanning electron microscope. DNA quantification was used to assess decellularisation. pMSCs were plated on scaffolds by static seeding and maintained in in vitro culture for 21 days. At 3, 7, 14 and 21 days, seeded ECM scaffolds were evaluated for cellular adhesion and growth. Moreover, the expression of specific hepatic genes was performed by RT-PCR.

Results: The applied decellularisation/recellularisation protocol was effective. The number of seeded pMSCs increased over the culture time points. Gene expression analysis of seeded pMSCs displayed a weak induction due to ECM towards HLCs.

Conclusions: These results suggest that ECM may address pMSCs to differentiate in hepatocyte-like cells. However, only contact with liver-ECM is not enough to induce complete differentiation.

  • Bioscaffold
  • Decellularisation
  • Extracellular matrix
  • Mesenchymal stromal cells
  • Recellularisation
  • Tissue engineering
Citation (ISO format)
CROCE, Stefania et al. Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering. In: Biomedicines, 2022, vol. 10, n° 11, p. 2817. doi: 10.3390/biomedicines10112817
Main files (1)
Article (Published version)
Secondary files (7)
ISSN of the journal2227-9059

Technical informations

Creation11/21/2022 1:48:00 PM
First validation11/21/2022 1:48:00 PM
Update time03/16/2023 10:33:13 AM
Status update03/16/2023 10:33:09 AM
Last indexation08/31/2023 10:32:14 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack