Scientific article
Open access

Ultrasensitive immunoassay allows measurement of serum neurofilament heavy in multiple sclerosis

Published inMultiple sclerosis and related disorders, vol. 50, 102840
Publication date2021-05
First online date2021-02-10

Background: Neurofilament heavy (NfH) is a promising biomarker for neuro-axonal damage in Multiple Sclerosis (MS). We compared the performance of high-sensitivity serum-NfH immunoassays, with as aim to investigate the value of serum-NfH as biomarker for MS.

Methods: We measured serum-NfH in 76 MS patients with Simoa (one commercial, one in-house) or Luminex assays. Serum-NfH measured by the immunoassay with greatest sensitivity was related to clinical and radiological outcomes with age and sex-adjusted linear regression analysis, and to biological outcomes cerebrospinal fluid (CSF)-NfH, serum neurofilament light (NfL) and CSF-NfL with Spearman's correlation analysis.

Results: With the commercial Simoa assay, we obtained 100% serum-NfH detectability (in-house Simoa: 70%, Luminex: 61%), with lowest coefficient of variation (CV) between duplicates of 11%CV (in-house Simoa: 22%CV, Luminex: 30%CV). Serum-NfH quantified with the commercial Simoa assay was associated with disease duration (standardized beta (sβ) = 0.28, p = 0.034), T2 lesion volume (sβ = 0.23, p = 0.041), and tended to associate with black hole count (sβ = 0.21, p = 0.084) but not with Expanded Disease Disability Score (EDSS) or normalized brain volume (all: p>0.10). Furthermore, serum-NfH showed correlations with CSF-NfH (rho = 0.27, p = 0.018) and serum-NfL (rho=0.44, p < 0.001), but not with CSF-NfL.

Conclusions: Serum-NfH can be quantified with high-sensitivity technology. Cross-sectionally, we observed some weak correlations of serum-NfH with MS disease burden parameters, suggesting there might be some utility for serum-NfH as biomarker for MS disease burden.

  • Blood biomarker
  • Multiple sclerosis
  • Neurofilament heavy
  • Neurofilament light
  • Simoa
  • Biomarkers
  • Humans
  • Immunoassay
  • Intermediate Filaments
  • Multiple Sclerosis / diagnostic imaging
  • Neurofilament Proteins
Citation (ISO format)
VERBERK, Inge M W et al. Ultrasensitive immunoassay allows measurement of serum neurofilament heavy in multiple sclerosis. In: Multiple sclerosis and related disorders, 2021, vol. 50, p. 102840. doi: 10.1016/j.msard.2021.102840
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ISSN of the journal2211-0356

Technical informations

Creation12/22/2022 1:46:00 PM
First validation12/22/2022 1:46:00 PM
Update time03/16/2023 10:26:04 AM
Status update03/16/2023 10:26:03 AM
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