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Scientific article
Open access
English

Systems analysis of human responses to an aluminium hydroxide-adsorbed TLR7 agonist (AS37) adjuvanted vaccine reveals a dose-dependent and specific activation of the interferon-mediated antiviral response

Published inVaccine, vol. 41, no. 3, p. 724-734
Publication date2023-01-16
First online date2022-12-21
Abstract

The candidate Adjuvant System AS37 contains a synthetic toll-like receptor agonist (TLR7a) adsorbed to alum. In a phase I study (NCT02639351), healthy adults were randomised to receive one dose of licensed alum-adjuvanted meningococcal serogroup C (MenC-CRM197) conjugate vaccine (control) or MenC-CRM197conjugate vaccine adjuvanted with AS37 (TLR7a dose 12.5, 25, 50 or 100 µg). A subset of 66 participants consented to characterisation of peripheral whole blood transcriptomic responses, systemic cytokine/chemokine responses and multiple myeloid and lymphoid cell responses as exploratory study endpoints. Blood samples were collected pre-vaccination, 6 and 24 h post-vaccination, and 3, 7, 28 and 180 days post-vaccination. The gene expression profile in whole blood showed an early, AS37-specific transcriptome response that peaked at 24 h, increased with TLR7a dose up to 50 µg and generally resolved within one week. Five clusters of differentially expressed genes were identified, including those involved in the interferon-mediated antiviral response. Evaluation of 30 cytokines/chemokines by multiplex assay showed an increased level of interferon-induced chemokine CXCL10 (IP-10) at 24 h and 3 days post-vaccination in the AS37-adjuvanted vaccine groups. Increases in activated plasmacytoid dendritic cells (pDC) and intermediate monocytes were detected 3 days post-vaccination in the AS37-adjuvanted vaccine groups. T follicular helper (Tfh) cells increased 7 days post-vaccination and were maintained at 28 days post-vaccination, particularly in the AS37-adjuvanted vaccine groups. Moreover, most of the subjects that received vaccine containing 25, 50 and 100 µg TLR7a showed an increased MenC-specific memory B cell responses versus baseline. These data show that the adsorption of TLR7a to alum promotes an immune signature consistent with TLR7 engagement, with up-regulation of interferon-inducible genes, cytokines and frequency of activated pDC, intermediate monocytes, MenC-specific memory B cells and Tfh cells. TLR7a 25-50 µg can be considered the optimal dose for AS37, particularly for the adjuvanted MenC-CRM197conjugate vaccine.

eng
Keywords
  • Adjuvant System
  • Immune response
  • Plasmacytoid dendritic cells
  • Toll-like receptor 7
  • Type I interferon
  • Vaccine
  • Adult
  • Humans
  • Aluminum Hydroxide
  • Interferons
  • Toll-Like Receptor 7
  • Antiviral Agents
  • Vaccines, Conjugate
  • Meningococcal Vaccines
  • Adjuvants, Immunologic
  • Cytokines
  • Systems Analysis
Affiliation Not a UNIGE publication
Citation (ISO format)
SIENA, Emilio et al. Systems analysis of human responses to an aluminium hydroxide-adsorbed TLR7 agonist (AS37) adjuvanted vaccine reveals a dose-dependent and specific activation of the interferon-mediated antiviral response. In: Vaccine, 2023, vol. 41, n° 3, p. 724–734. doi: 10.1016/j.vaccine.2022.12.006
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Article (Published version)
Identifiers
ISSN of the journal0264-410X
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Technical informations

Creation01/05/2023 9:37:00 PM
First validation01/05/2023 9:37:00 PM
Update time03/16/2023 10:23:16 AM
Status update03/16/2023 10:23:16 AM
Last indexation02/01/2024 9:23:30 AM
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