In soccer, physical development is crucial for developing optimal performance. This study aimed to assess and compare the physical development of elite and non-elite professional soccer players. Seventy-eight male professional football players divided into four competitive levels participated in this study: the elite group (EG), the non-elite group A (NEG-A), the non-elite group B (NEG-B), and the under 23 group (U23). Body composition, static strength, lower-body explosive strength, flexibility, and balance were assessed. No significant statistical differences between elite and non-elite players were seen in body composition parameters. However, the EG performed better in static strength, lower-body explosive strength, flexibility, and balance, even after adjusting for the effects of chronological age. The analysis showed that the competitive level (group) explained 25% to 29% of the variance observed in the lower-body explosive strength tasks. Sports staff and coaches in different age categories or competitive levels should include specific lower-body explosive strength content during soccer training to promote players’ long-term development towards the elite level.