en
Scientific article
Open access
English

An autoinhibitory clamp of actin assembly constrains and directs synaptic endocytosis

Published ineLife, vol. 10, e69597
Publication date2021-07-29
First online date2021-07-29
Abstract

Synaptic membrane-remodeling events such as endocytosis require force-generating actin assembly. The endocytic machinery that regulates these actin and membrane dynamics localizes at high concentrations to large areas of the presynaptic membrane, but actin assembly and productive endocytosis are far more restricted in space and time. Here we describe a mechanism whereby autoinhibition clamps the presynaptic endocytic machinery to limit actin assembly to discrete functional events. We found that collective interactions between the Drosophila endocytic proteins Nwk/FCHSD2, Dap160/intersectin, and WASp relieve Nwk autoinhibition and promote robust membrane-coupled actin assembly in vitro. Using automated particle tracking to quantify synaptic actin dynamics in vivo, we discovered that Nwk-Dap160 interactions constrain spurious assembly of WASp-dependent actin structures. These interactions also promote synaptic endocytosis, suggesting that autoinhibition both clamps and primes the synaptic endocytic machinery, thereby constraining actin assembly to drive productive membrane remodeling in response to physiological cues.

eng
Keywords
  • D. melanogaster
  • Actin
  • Cell biology
  • Endocytosis
  • Neuroscience
  • Synapse
Research group
Funding
Citation (ISO format)
DEL SIGNORE, Steven J et al. An autoinhibitory clamp of actin assembly constrains and directs synaptic endocytosis. In: eLife, 2021, vol. 10, p. e69597. doi: 10.7554/eLife.69597
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal2050-084X
115views
60downloads

Technical informations

Creation11/21/2022 6:12:00 PM
First validation11/21/2022 6:12:00 PM
Update time03/16/2023 9:06:26 AM
Status update03/16/2023 9:06:25 AM
Last indexation02/01/2024 9:13:54 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack