en
Scientific article
Open access
English

Influence of needle design and irrigant flow rate on the removal of enterococcus faecalis biofilms in vitro

Published inDentistry journal, vol. 10, no. 4, 59
Publication date2022-04-02
First online date2022-04-02
Abstract

This study aimed to evaluate the influence of needle design and irrigant flow rate on the removal of Enterococcus faecalis mature biofilms during sodium hypochlorite irrigation. Forty-eight single-rooted human teeth were instrumented (ProTaper F3), autoclaved and inoculated with Enterococcus faecalis to establish a two-week-old biofilm. E. faecalis biofilms were treated with Sodium hypochlorite that was injected in the root canals using three types of needles (NaviTip, ProRinse, IrriFlex). For the IrriFlex needle, one, two, or four bars of pressure was applied to the irrigating solution to increase flow rates. Bacteria were labeled with the LIVE/DEAD BacLight Bacterial Viability kit, and viability was assessed by flow cytometry (FCM). Results were statistically analyzed using one-way ANOVA and Tukey multiple comparison intervals (α = 0.05). Bacterial viability was significantly reduced after sodium hypochlorite passive irrigation but the number of viable bacteria retrieved from root canal specimens irrigated with the Pro-Rinse needle was significantly higher compared to NaviTip and IrriFlex needles (p < 0.05). When the irrigant flow rate was increased, the viability of bacterial biofilms was significantly reduced compared to passive irrigation using the IrriFlex needle (p < 0.05). Applying higher flow rates during irrigation using the IrriFlex needle did not further reduce bacterial viability.

eng
Keywords
  • E. faecalis biofilms
  • Endodontic irrigation
  • Needle
Citation (ISO format)
PROVOOST, Charley et al. Influence of needle design and irrigant flow rate on the removal of enterococcus faecalis biofilms in vitro. In: Dentistry journal, 2022, vol. 10, n° 4, p. 59. doi: 10.3390/dj10040059
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal2304-6767
109views
37downloads

Technical informations

Creation11/28/2022 1:53:00 PM
First validation11/28/2022 1:53:00 PM
Update time03/16/2023 9:05:55 AM
Status update03/16/2023 9:05:55 AM
Last indexation02/01/2024 9:13:41 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack