en
Scientific article
Open access
English

Development and application of a liquid chromatography coupled to mass spectrometry method for the simultaneous determination of 23 antineoplastic drugs at trace levels

Publication date2022-11-30
First online date2022-09-08
Abstract

The goal of this study was to develop a method for the simultaneous quantification of 23 commonly used antineoplastic drugs in a hospital pharmacy, using ultra-high pressure liquid chromatography separation coupled to tandem mass spectrometry detection (UHPLC-MS/MS). The following drugs were investigated: 5-fluorouracil, cytarabine, ganciclovir, gemcitabine, dacarbazine, methotrexate, pemetrexed, busulfan, topotecan, rentitrexed, ifosfamide, cyclophosphamide, etoposide, irinotecan, doxorubicin/epirubicin, vincristine, docetaxel, paclitaxel, daunorubicin, idarubicin, vinblastine, oxaliplatin and carboplatin. The chromatographic separation was performed on a phenyl-hexyl column (2.1 ×100 mm, 1.7 µm) with a gradient elution of methanol and water containing 10 mM ammonium formate adjusted to pH 4.9. All compounds were analyzed in less than 13 min and detected with a triple quadrupole mass spectrometer operating in MRM mode. Limits of detection (LODs) and limits of quantification (LOQs) were comprised between 0.01 and 5 ng.mL-1, and between 0.5 and 5 ng.mL-1, respectively. Accuracies ranged between 117% and 83% at the LOQ, intermediate and upper LOQ concentrations, with relative standard deviations (RSD) inferior to 8%, for all the antineoplastic drugs. Finally, the UHPLC-MS/MS method was successfully applied to the analysis of surface samples to evaluate the chemical contamination by these highly toxic compounds in a chemotherapy preparation unit in a hospital pharmacy with the purpose of monitoring the exposure of health care professionals.

eng
Keywords
  • Antineoplastic drug
  • Mass spectrometry
  • Reversed phase liquid chromatography
  • Surface analyses
  • Trace analysis
Citation (ISO format)
FLEURY, Sandrine et al. Development and application of a liquid chromatography coupled to mass spectrometry method for the simultaneous determination of 23 antineoplastic drugs at trace levels. In: Journal of pharmaceutical and biomedical analysis, 2022, vol. 221, p. 115034. doi: 10.1016/j.jpba.2022.115034
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal0731-7085
105views
46downloads

Technical informations

Creation10/18/2022 12:40:00 PM
First validation10/18/2022 12:40:00 PM
Update time03/16/2023 9:02:59 AM
Status update03/16/2023 9:02:58 AM
Last indexation02/01/2024 9:11:37 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack