en
Scientific article
English

Endosomal fractions from viral K-ras-transformed MDCK cells reveal transformation specific changes on two-dimensional gel maps

Published inElectrophoresis, vol. 17, no. 11, p. 1734-1740
Publication date1996
Abstract

We have investigated the effects of viral Kirsten ras oncogene expression in Madin-Darby canine kidney (MDCK) II epithelial cell on the differential protein expression of organelle proteins. MDCK cells, stably transformed via infection with a helper-independent retroviral vector construct, were grown on permeable filter supports. Whereas normal cells form highly polarized monolayers, ras-transformed cells display an unpolarized phenotype, detaching from the substratum and developing multilayers (Schoenenberger, C.-A. et al., J. Cell Biol. 1991, 112, 873–889). We postulate that this breakdown of epithelial polarity reflects disturbed intracellular protein transport and sorting, namely, proteins will no longer be sorted correctly in intracellular organelles and will therefore not reach their appropriate target membrane. Here we emphasize the role of endosomes as sorting platform in epithelial cells. We found significant differences in the molecular composition of endosomes from normal vs. oncogenic transformed epithelial cells, strengthening previous evidence indicating that oncogenic transformation results in abnormal expression of normal genes (Celis, J. E., Olsen, E., Electrophoresis 1994, 15, 309–344) as well as the expression of new ones (Huber, L. A. et al., Electrophoresis 1994, 15, 468–473).

eng
Keywords
  • Endosomes
  • Two-dimensional polyacrylamide gel electrophoresis
  • K-ras
  • Transformation
  • Epithelium
Citation (ISO format)
HUBER, Lukas et al. Endosomal fractions from viral K-<i>ras</i>-transformed MDCK cells reveal transformation specific changes on two-dimensional gel maps. In: Electrophoresis, 1996, vol. 17, n° 11, p. 1734–1740. doi: 10.1002/elps.1150171111
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0173-0835
95views
0downloads

Technical informations

Creation11/18/2022 10:56:00 AM
First validation11/18/2022 10:56:00 AM
Update time03/16/2023 9:02:47 AM
Status update03/16/2023 9:02:46 AM
Last indexation01/17/2024 10:18:20 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack