en
Scientific article
Open access
English

A Metabolomic Signature of Acute Caloric Restriction

Published inThe Journal of clinical endocrinology and metabolism, vol. 102, no. 12, p. 4486-4495
Publication date2017-12-01
First online date2017-09-28
Abstract

Context: The experimental paradigm of acute caloric restriction (CR) followed by refeeding (RF) can be used to study the homeostatic mechanisms that regulate energy homeostasis, which are relevant to understanding the adaptive response to weight loss.

Objective: Metabolomics, the measurement of hundreds of small molecule metabolites, their precursors, derivatives, and degradation products, has emerged as a useful tool for the study of physiology and disease and was used here to study the metabolic response to acute CR.

Participants, design, and setting: We used four ultra high-performance liquid chromatography-tandem mass spectrometry methods to characterize changes in carbohydrates, lipids, amino acids, and steroids in eight normal weight men at baseline, after 48 hours of CR (10% of energy requirements) and after 48 hours of ad libitum RF in a tightly controlled environment.

Results: We identified a distinct metabolomic signature associated with acute CR characterized by the expected switch from carbohydrate to fat utilization with increased lipolysis and β-fatty acid oxidation. We found an increase in ω-fatty acid oxidation and levels of endocannabinoids, which are known to promote food intake. These changes were reversed with RF. Several plasmalogen phosphatidylethanolamines (endogenous antioxidants) significantly decreased with CR (all P ≤ 0.0007). Additionally, acute CR was associated with an increase in the branched chain amino acids (all P ≤ 1.4 × 10-7) and dehydroepiandrosterone sulfate (P = 0.0006).

Conclusions: We identified a distinct metabolomic signature associated with acute CR. Further studies are needed to characterize the mechanisms that mediate these changes and their potential contribution to the adaptive response to dietary restriction.

eng
Keywords
  • Adult
  • Amino Acids / metabolism
  • Antioxidants / metabolism
  • Caloric Restriction
  • Carbohydrate Metabolism / physiology
  • Chromatography, High Pressure Liquid
  • Dehydroepiandrosterone Sulfate / metabolism
  • Endocannabinoids / metabolism
  • Energy Metabolism / physiology
  • Fatty Acids / metabolism
  • Homeostasis
  • Humans
  • Lipid Metabolism / physiology
  • Lipolysis
  • Male
  • Metabolomics / methods
  • Oxidation-Reduction
  • Steroids / blood
  • Tandem Mass Spectrometry
  • Weight Loss / physiology
Affiliation Not a UNIGE publication
Research group
Funding
Citation (ISO format)
COLLET, Tinh-Hai et al. A Metabolomic Signature of Acute Caloric Restriction. In: The Journal of clinical endocrinology and metabolism, 2017, vol. 102, n° 12, p. 4486–4495. doi: 10.1210/jc.2017-01020
Main files (1)
Article (Published version)
Secondary files (2)
Identifiers
ISSN of the journal0021-972X
76views
31downloads

Technical informations

Creation04/02/2022 5:37:00 AM
First validation04/02/2022 5:37:00 AM
Update time03/16/2023 8:58:11 AM
Status update03/16/2023 8:58:07 AM
Last indexation05/06/2024 2:36:51 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack