Repetitive activation of the mesolimbic dopamine system by drugs or optogenetic dopamine neuron self-stimulation can lead to compulsive reinforcement-seeking behaviors. The most addictive substances such as cocaine/amphetamines lead to 10-20% of consumers eventually developing an addiction. However, little is known about individual vulnerability to addiction, mainly because investigations have focused on brain changes after drug exposure due to a lack of technology to study the biological underpinning of addiction in the same animal before and after its exposure to drugs/addictive protocols. Here we showed that this compulsive reinforcement, a characterizing symptom of addiction, is related to an alteration of the orbitofrontal cortex to Dorsal striatum transmission. Then we developed a methodological pipeline combining brain tissue imprinting and sequencing of that brain tissue. Such a method will allow future investigations aiming at identifying addiction risk factors by correlating the gene expression of each naïve mouse with its own behavior during oDASS.