Doctoral thesis

Brain Micro-Biopsies for in Vivo Longitudinal Investigation of Compulsion in a Model of Addiction

ContributorsAchargui, Ridouane
Imprimatur date2022-07-04

Repetitive activation of the mesolimbic dopamine system by drugs or optogenetic dopamine neuron self-stimulation can lead to compulsive reinforcement-seeking behaviors. The most addictive substances such as cocaine/amphetamines lead to 10-20% of consumers eventually developing an addiction. However, little is known about individual vulnerability to addiction, mainly because investigations have focused on brain changes after drug exposure due to a lack of technology to study the biological underpinning of addiction in the same animal before and after its exposure to drugs/addictive protocols. Here we showed that this compulsive reinforcement, a characterizing symptom of addiction, is related to an alteration of the orbitofrontal cortex to Dorsal striatum transmission. Then we developed a methodological pipeline combining brain tissue imprinting and sequencing of that brain tissue. Such a method will allow future investigations aiming at identifying addiction risk factors by correlating the gene expression of each naïve mouse with its own behavior during oDASS.

  • Neuroscience
  • Data analysis
  • Psychology
  • Drugs
  • Bio-engineering
  • Product Development
  • Medical device
Citation (ISO format)
ACHARGUI, Ridouane. Brain Micro-Biopsies for in Vivo Longitudinal Investigation of Compulsion in a Model of Addiction. 2022. doi: 10.13097/archive-ouverte/unige:164531
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Technical informations

Creation11/01/2022 10:43:00 AM
First validation11/01/2022 10:43:00 AM
Update time03/16/2023 8:37:01 AM
Status update03/16/2023 8:36:59 AM
Last indexation02/01/2024 9:01:39 AM
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