BACKGROUND AND PURPOSE: Controversy exists as to whether ADC histograms are capable to distinguish human papillomavirus-positive (HPV+) from human papillomavirus-negative (HPV-) oropharyngeal squamous cell carcinoma. We investigated how the choice of b-values influences the capability of ADC histograms to distinguish between the two tumor types.

MATERIALS AND METHODS: Thirty-four consecutive patients with histologically proved primary oropharyngeal squamous cell carcinoma (11 HPV+ and 23 HPV-) underwent 3T MR imaging with a single-shot EPI DWI sequence with 6 b-values (0, 50, 100, 500, 750, 1000 s/mm²). Monoexponentially calculated perfusion-sensitive (including b=0 s/mm²) and perfusion-insensitive/true diffusion ADC maps (with b ≥ 100 s/mm²as the lowest b-value) were generated using Matlab. The choice of b-values included 2 b-values (ADC_b0-1000, ADC_b100-1000, ADC_b500-1000, ADC_b750-1000) and 3-6 b-values (ADC_b0-750-1000, ADC_{b0-500-750-1000}, ADC_{b0-50-100-1000}, ADC_{b0-50-100-750-1000}, ADC_{b0-50-100-500-750-1000}). Readers blinded to the HPV- status contoured all tumors. ROIs were then copied onto ADC maps, and their histograms were compared.

RESULTS: ADC histogram metrics in HPV+ and HPV- oropharyngeal squamous cell carcinoma changed significantly depending on the b-values. The mean ADC was lower, and skewness was higher in HPV+ than in HPV- oropharyngeal squamous cell carcinoma only for ADC_b0-1000,ADC_b0-750-1000, and ADC_{b0-500-750-1000}(P< .05), allowing distinction between the 2 tumor types. Kurtosis was significantly higher in HPV+ versus HPV- oropharyngeal squamous cell carcinoma for all b-value combinations except 2 perfusion-insensitive maps (ADC_b500-1000and ADC_b750-1000). Among all b-value combinations, kurtosis on ADC_b0-1000had the highest diagnostic performance to distinguish HPV+ from HPV- oropharyngeal squamous cell carcinoma (area under the curve = 0.893; sensitivity = 100%, specificity = 82.6%). Acquiring multiple b-values for ADC calculation did not improve the distinction between HPV+ and HPV- oropharyngeal squamous cell carcinoma.

CONCLUSIONS: The choice of b-values significantly affects ADC histogram metrics in oropharyngeal squamous cell carcinoma. Distinguishing HPV+ from HPV- oropharyngeal squamous cell carcinoma is best possible on the ADC_b0-1000map.