Scientific article
English

Isolation and characterisation of a Bam HI element in psam 3 a gene of Pisum sativum L. induced during early stages of arbuscular mycorrhiza development

Published inJournal of plant physiology, vol. 158, no. 2, p. 261-266
Publication date2001-01
Abstract

An Alu-like element was identified in the 5′-UTR of psam 3, a Pisum sativum L. gene which shows enhanced expression during early events of AM formation. Two sets of primers specific for the 5′-UTR of the gene psam 3 and for the Alu-like element, respectively, were designed to study psam 3 gene organisation by targeted Alu PCR carried out on pea genomic DNA. PCR products free of Alu-like sequences were produced. A 1.0 kb DNA fragment showing up to 65 percnt; similarity to a Bam HI repeated sequence of Vicia faba was isolated in both wild-type and mycorrhiza-resistant pea. Southern blot analysis revealed the presence of other Bam HI related sequences in pea. The possibility that Bam HI repeated sequences might constitute complex repeating units together with an Alu-like element in the P. sativum genome is discussed.

Keywords
  • Arbuscular mycorrhiza
  • Alu-like element
  • Repeated sequence
  • Pisum sativum L.
  • Glomus mosseae
Affiliation entities Not a UNIGE publication
Citation (ISO format)
MARTIN-LAURENT, Fabrice et al. Isolation and characterisation of a Bam HI element in psam 3 a gene of Pisum sativum L. induced during early stages of arbuscular mycorrhiza development. In: Journal of plant physiology, 2001, vol. 158, n° 2, p. 261–266. doi: 10.1078/0176-1617-00113
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Journal ISSN0176-1617
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