The purpose of this paper is to demonstrate the complementary utility of chlorophyll fast fluorescence OJIP transient (from 50 μs to 1 s) measurements in the aerial study of rows of trees. We identify limitations in photochemical events induced by urban injuries on Platanus acerfolia L., using the JIP-test procedure. The Performance Index (PI_ABS) showed the largest dynamic range to characterise the vitality of trees. Individual trees were graded into three quality groups based on the individual PI_ABS value compared to the overall average for trees in the alley. These groups are: high performers, with PI_ABS 50 percnt; higher than the alley average; normal trees, with a deviation from the alley average between-50 percnt; and +50 percnt;; and poor performers, whose deviation from the alley average was-50 percnt; or less. Trees also were grouped into five vitality categories on the basis of a stereoscopic and morphologic observation of the symmetry of tree crowns, percentage of defoliation and reflectance property in the visible and infra-red range. Here, we report a remarkable correlation between the airborne remote sensing data and the on-site fluorescence measurements.