Scientific article

Photosynthetic response of transgenic soybean plants, containing an Arabidopsis P5CR gene, during heat and drought stress

Published inJournal of plant physiology, vol. 161, no. 11, p. 1211-1224
Publication date2004-11

The biochemical basis of heat/drought tolerance was investigated by comparing the response of antisense and sense transgenic soybean plants (containing the l-Δ1-pyrroline-5-carboxylate reductase gene) with non-transgenic wild-type plants. The plants were subjected to a simultaneous drought and heat stress of 2 days, whereafter they were rewatered at 25°C. During this time the sense plants only showed mild symptoms of stress compared to the antisense plants which were severely stressed. Upon stress, nicotinamide adenine dinucleotide phosphate (NADP+) levels decreased in antisense while it increased in sense plants. Recovery with respect to NADP+ levels was best in sense plants. Sense plants had the highest ability to accumulate proline during stress and to metabolise proline after rewatering. Analyses of the fast phase chlorophyll-a fluorescence transients showed dissociation of the oxygen-evolving complex (OEC) upon stress in all plants tested. In the sense plants, which best resisted the stress, OEC dissociation was bypassed by proline feeding electrons into photosystem 2 (PSII), maintaining an acceptable nicotinamide adenine dinucleotide hydrogen phosphate (NADPH) level, preventing further damage. Upon recovery, NADPH is consumed during oxidation of accumulated proline providing high levels of NADP+ to act as electron acceptor to PSII, which indirectly may ameliorate the inhibition and/or the effect of uncoupling of the OEC.

  • Chlorophyll fluorescence
  • Drought
  • Heat
  • NADP+
  • Proline
  • Soybean
Citation (ISO format)
DE RONDE, J.A. et al. Photosynthetic response of transgenic soybean plants, containing an <i>Arabidopsis</i> <i>P</i>5<i>CR</i> gene, during heat and drought stress. In: Journal of plant physiology, 2004, vol. 161, n° 11, p. 1211–1224. doi: 10.1016/j.jplph.2004.01.014
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Article (Published version)
ISSN of the journal0176-1617

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