Scientific article
Open access

Functional genetics in Apicomplexa: Potentials and limits

Published inFEBS letters, vol. 585, no. 11, p. 1579-1588
Publication date2011

The Apicomplexans are obligate intracellular protozoan parasites and the causative agents of severe diseases in humans and animals. Although complete genome sequences are available since many years and for several parasites, they are replete with putative genes of unassigned function. Forward and reverse genetic approaches are limited only to a few Apicomplexans that can either be propagated in vitro or in a convenient animal model. This review will compare and contrast the most recent strategies developed for the genetic manipulation of Plasmodium falciparum, Plasmodium berghei and Toxoplasma gondii that have taken advantage of the intrinsic features of their respective genomes. Efforts towards the improvement of the transfection efficiencies in malaria parasites, the development of approaches to study essential genes and the elaboration of high-throughput methods for the identification of gene function will be discussed.

Citation (ISO format)
LIMENITAKIS, Julien, SOLDATI-FAVRE, Dominique. Functional genetics in Apicomplexa: Potentials and limits. In: FEBS letters, 2011, vol. 585, n° 11, p. 1579–1588. doi: 10.1016/j.febslet.2011.05.002
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Article (Accepted version)
ISSN of the journal0014-5793

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