Muscle stem cells (MuSCs) are essential cells allowing muscle regeneration following injury. The fate of MuSCs is finely regulated by transcriptional modifications allowing metabolic flexibility to finally establish a balance between quiescence and activation. In pathologies such as Duchenne muscular dystrophy, there is a disturbance of the MuSC niche, which may impact the regenerative capacities of MuSCs. So far, the metabolism of MuSCs has been studied only after isolation of MuSCs from their niche, even though the latter seems to be crucial for efficient muscle repair. In order to obtain a picture as close as possible to physiology, I used the technique developed by my supervisor A. Prola to study the enzymatic metabolism of MuSCs within tissues of dystrophic and wild-type mice.