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Scientific article
Open access
English

DNA Vaccine Administered by Cationic Lipoplexes or by In Vivo Electroporation Induces Comparable Antibody Responses against SARS-CoV-2 in Mice

Published inVaccines, vol. 9, no. 8, 874
Publication date2021-08-06
First online date2021-08-06
Abstract

In view of addressing the global necessity of an effective vaccine in the SARS-CoV-2 pandemic, a plasmid DNA vaccine, expressing for the spike (S) protein and formulated in lipoplexes, was manufactured and tested for in vitro transfection and in vivo immunogenicity. Blank cationic liposomes of 130.9 ± 5.8 nm in size and with a zeta potential of +48 ± 12 mV were formulated using the thin-film layer rehydration method. Liposomes were complexed with pCMVkan-S at different N/P ratios. Ratios of 0.25:1 and 1:1 were selected according to their complex stability and controlled size compared to other ratios and tested in vitro for transfection studies and in vivo for immunogenicity. Both selected formulations showed enhanced neutralizing antibody responses compared to pCMVkan-S injected alone, as well as an increased T cell response. The titers observed were similar to those of intramuscular electroporation (IM-EP), which was set as an efficacy goal.

Keywords
  • DNA vaccines
  • SARS-CoV-2
  • Cationic liposomes
  • Immunogenicity
  • Lipoplexes
Research group
Funding
  • National Vaccine Institute - [DNS 63_031_30_009_2]
  • National Vaccine Institute (NVI) - [2563.1/8]
  • Chulalongkorn University and Ratchadapiseksompotch Fund, Faculty of Medicine, Chulalongkorn University - [RA-MF-28/64]
Citation (ISO format)
PELETTA, Allegra et al. DNA Vaccine Administered by Cationic Lipoplexes or by In Vivo Electroporation Induces Comparable Antibody Responses against SARS-CoV-2 in Mice. In: Vaccines, 2021, vol. 9, n° 8, p. 874. doi: 10.3390/vaccines9080874
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Article (Published version)
Identifiers
ISSN of the journal2076-393X
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Technical informations

Creation01/13/2022 1:46:00 PM
First validation01/13/2022 1:46:00 PM
Update time03/16/2023 2:22:55 AM
Status update03/16/2023 2:22:53 AM
Last indexation05/06/2024 8:53:01 AM
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