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Scientific article
Open access
English

Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage

Publication date2021
Abstract

In the context of an unprecedented shortage of nasopharyngeal swabs (NPS) or sample transport media during the coronavirus disease 2019 (COVID-19) crisis, alternative methods for sample collection are needed. To address this need, we validated a cell culture medium as a viral transport medium, and compared the analytical sensitivity of SARS-CoV-2 RT-PCR in nasal wash (NW), oropharyngeal swab (OPS), and NPS specimens. Both the clinical and analytical sensitivity were comparable in these three sample types. OPS and NW specimens may therefore represent suitable alternatives to NPS for SARS-CoV-2 detection.

Keywords
  • COVID-19/diagnosis
  • Culture Media
  • Humans
  • Nasopharynx/virology
  • Nose/virology
  • Oropharynx/virology
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • SARS-CoV-2/isolation & purification
  • Specimen Handling/methods
Citation (ISO format)
CALAME, Adrien et al. Sensitivity of nasopharyngeal, oropharyngeal, and nasal wash specimens for SARS-CoV-2 detection in the setting of sampling device shortage. In: European Journal of Clinical Microbiology & Infectious Diseases, 2021, vol. 40, n° 2, p. 441–445. doi: 10.1007/s10096-020-04039-8
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Article (Published version)
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Identifiers
ISSN of the journal0934-9723
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Technical informations

Creation09/20/2021 11:23:00 AM
First validation09/20/2021 11:23:00 AM
Update time03/16/2023 1:59:51 AM
Status update03/16/2023 1:59:50 AM
Last indexation02/12/2024 12:16:07 PM
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