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Scientific article
Open access
English

High levels of cellular retinol binding protein-1 expression in leiomyosarcoma: possible implications for diagnostic evaluation

Published inVirchows Archiv, vol. 441, no. 1, p. 31-40
Publication date2002
Abstract

Retinoid bioavailability is regulated by the activity of specific cytoplasmic receptors. High levels of cellular retinol binding protein-1 (CRBP-1) have been documented during experimental arterial and wound-healing processes, but data concerning neoplastic smooth muscle tissues are scarce and/or controversial. This study reports that the expression of CRBP-1 is markedly higher in uterine and gastrointestinal leiomyosarcomas than in leiomyomas and normal myometrium; CRBP-1 was practically absent in normal gastrointestinal smooth muscle tissue. CRBP-1 positivity was particularly elevated in the epithelioid variant of leiomyosarcoma; it was associated with increased proliferative and apoptotic rates and inversely related to smooth muscle differentiation evaluated by alpha- and gamma-smooth muscle actin and desmin expression. Western blotting and reverse-transcription polymerase chain reaction confirmed the observations concerning CRBP-1 and actin isoform expression and revealed higher NF-kappa-Bp65 and RAR alpha and lower Bax protein levels in leiomyosarcoma than in the other conditions. These findings document that a high CRBP-1 expression is associated with smooth muscle malignancy and suggest that CRBP-1 expression represents a new useful marker for the classification of unusual variants of smooth muscle tumors.

Keywords
  • Adult
  • Aged
  • Biomarkers, Tumor
  • Female
  • Gastrointestinal Neoplasms/classification/diagnosis/metabolism
  • Humans
  • Leiomyosarcoma/classification/diagnosis/metabolism
  • Male
  • Middle Aged
  • Muscle, Smooth, Vascular/metabolism/pathology
  • Retinol-Binding Proteins/biosynthesis
  • Retinol-Binding Proteins, Cellular
  • Uterine Neoplasms/classification/diagnosis/metabolism
Citation (ISO format)
ORLANDI, Augusto et al. High levels of cellular retinol binding protein-1 expression in leiomyosarcoma: possible implications for diagnostic evaluation. In: Virchows Archiv, 2002, vol. 441, n° 1, p. 31–40. doi: 10.1007/s00428-001-0576-7
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Article (Published version)
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ISSN of the journal0945-6317
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