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Using Biotinylated myo-Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate–Protein Interactions with Surface-Based Biosensors

Published inBiochemistry, vol. 60, no. 37, p. 2739-2748
Publication date2021
Abstract

Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins to regulate biological functions, such as protein-protein interactions. PP-InsPs are strongly negatively charged and interact with highly basic surface patches in proteins, making their quantitative biochemical analysis challenging. Here, we present the synthesis of biotinylated myo-inositol hexakisphosphates and their application in surface plasmon resonance and grating-coupled interferometry assays, to enable the rapid identification, validation, and kinetic characterization of InsP- and PP-InsP-protein interactions.

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Citation (ISO format)
COUTO, Daniel et al. Using Biotinylated <i>myo</i>-Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate–Protein Interactions with Surface-Based Biosensors. In: Biochemistry, 2021, vol. 60, n° 37, p. 2739–2748. doi: 10.1021/acs.biochem.1c00497
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ISSN of the journal0006-2960
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Creation09/20/2021 5:17:00 PM
First validation09/20/2021 5:17:00 PM
Update time03/16/2023 1:22:15 AM
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