Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real-time PCR and real-time-PCR associated to propidium monoazide

Sereti, Maria; Zekeridou, Alkisti; Cancela, José Antonio; Mombelli, Andrea; Giannopoulou, Catherine

doi:10.1002/cre2.464

Scientific article

English

Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real-time PCR and real-time-PCR associated to propidium monoazide

ContributorsSereti, Maria

; Zekeridou, Alkisti; Cancela, José Antonio; Mombelli, Andrea; Giannopoulou, Catherine

Published inClinical and Experimental Dental Research

Publication date2021

Abstract

The aim of the present methodological study was to evaluate the discrepancies in the detection of a number of periodontally involved pathogenic bacteria obtained from clinical samples by two methods: the quantitative Polymerase Chain Reaction (qPCR) and the qPCR combined with pre-treatment by Propidium Monoazide (PMA).

Keywords

RT-PCR
Bacteria
Periodontal treatment
Plaque
Propidium monoazide

Affiliation entities

Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de paradontologie

Research groups

Groupe Giannopoulou Catherine (médecine dentaire) (626)

Citation (ISO format)

SERETI, Maria et al. Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real-time PCR and real-time-PCR associated to propidium monoazide. In: Clinical and Experimental Dental Research, 2021. doi: 10.1002/cre2.464

Article (Published version)

CC BY

Identifiers

PID : unige:154514
DOI : 10.1002/cre2.464
PMID : 34216116

Journal ISSN2057-4347

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134downloads

Creation01/09/2021 13:38:00

First validation01/09/2021 13:38:00

Update time16/03/2023 01:14:40

Status update16/03/2023 01:14:39

Last indexation31/10/2024 23:01:40

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Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real-time PCR and real-time-PCR associated to propidium monoazide

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