Background and objective: To compare measurements of a new retinal blood flow device with central retinal artery blood velocity. Materials and methods: One randomly selected eye from each of 13 subjects was examined. Blood flow was measured by laser blood flowmeter and velocity by color Doppler imaging. The correlation between measurements was analyzed using regression analysis where a P value of less than .05 and coefficient of regression values of greater than .5 were considered significant. Results: The laser blood flowmeter produced vessel diameter measurements of 90.1 +/- 18.7 microm (mean +/- standard deviation), velocity of 19.7 +/- 8.06 mm/sec, and flow measurements of 4.24 +/- 2.41 microL/min. Central retinal artery peak systolic velocity, end diastolic velocity, and mean velocity correlated significantly with laser blood flowmeter velocity (P = .01, r = .66; P < .01, r = .77; and P = .003, r = .76, respectively) and flow (P = .01, r = .71; P = .03, r = .6; and P = .01, r = .69, respectively). Conclusions: The laser blood flowmeter produces retinal artery flow and velocity measurements that correlate with central retinal artery peak systolic velocity end diastolic velocity, and mean velocity measurements. Further validation of the laser blood flowmeter's accuracy in measuring real flow warranted and likely requires more invasive in vivo studies (in animal models). However, this study supports the ability of the laser blood flowmeter to measure retinal blood flow.