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Title

Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A

Authors
Huch, Susanne
Polte, Christine
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Published in Cell Reports. 2021, vol. 36, no. 9, 109633
Abstract In this work, we show that Not4 and Not5 from the Ccr4-Not complex modulate translation elongation dynamics and change ribosome A-site dwelling occupancy in a codon-dependent fashion. These codon-specific changes in not5Δ cells are very robust and independent of codon position within the mRNA, the overall mRNA codon composition, or changes of mRNA expression levels. They inversely correlate with codon-specific changes in cells depleted for eIF5A and positively correlate with those in cells depleted for ribosome-recycling factor Rli1. Not5 resides in punctate loci, co-purifies with ribosomes and Rli1, but not with eIF5A, and limits mRNA solubility. Overexpression of wild-type or non-complementing Rli1 and loss of Rps7A ubiquitination enable Not4 E3 ligase-dependent translation of polyarginine stretches. We propose that Not4 and Not5 modulate translation elongation dynamics to produce a soluble proteome by Rps7A ubiquitination, dynamic condensates that limit mRNA solubility and exclude eIF5A, and a moonlighting function of Rli1.
Keywords Not1Not4Not5CondensatesTranslation elongationEIF5ARli1Codon optimality
Identifiers
PMID: 34469733
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Structures
Research group Caractérisation génétique et biochimique du complexe Ccr4-Not conservé (81)
Project
FNS: 31003A_172999
Citation
(ISO format)
ALLEN, George Edward et al. Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A. In: Cell Reports, 2021, vol. 36, n° 9, p. 109633. doi: 10.1016/j.celrep.2021.109633 https://archive-ouverte.unige.ch/unige:154342

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Deposited on : 2021-09-02

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