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Title

Single-cell transcriptomics of human and mouse lung cancers reveals conserved myeloid populations across individuals and species

Authors
Zilionis, Rapolas
Engblom, Camilla
Pfirschke, Christina
Savova, Virginia
Zemmour, David
Saatcioglu, Hatice D
Krishnan, Indira
Maroni, Giorgia
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Published in Immunity. 2019, vol. 50, no. 5, p. 1317-1334.e10
Abstract Tumor-infiltrating myeloid cells (TIMs) comprise monocytes, macrophages, dendritic cells, and neutrophils, and have emerged as key regulators of cancer growth. These cells can diversify into a spectrum of states, which might promote or limit tumor outgrowth but remain poorly understood. Here, we used single-cell RNA sequencing (scRNA-seq) to map TIMs in non-small-cell lung cancer patients. We uncovered 25 TIM states, most of which were reproducibly found across patients. To facilitate translational research of these populations, we also profiled TIMs in mice. In comparing TIMs across species, we identified a near-complete congruence of population structures among dendritic cells and monocytes; conserved neutrophil subsets; and species differences among macrophages. By contrast, myeloid cell population structures in patients' blood showed limited overlap with those of TIMs. This study determines the lung TIM landscape and sets the stage for future investigations into the potential of TIMs as immunotherapy targets.
Keywords AnimalsBase SequenceCarcinomaNon-Small-Cell Lung/immunology/pathologyCell LineTumorDendritic Cells/immunologyGene Expression ProfilingHumansLung/immunology/pathologyLung Neoplasms/immunology/pathologyMacrophages/immunologyMaleMiceMiceInbred C57BLMonocytes/immunologyNeutrophils/immunologySequence AnalysisRNA
Identifiers
PMID: 30979687
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Research group Groupe Mikael Pittet (1024)
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ZILIONIS, Rapolas et al. Single-cell transcriptomics of human and mouse lung cancers reveals conserved myeloid populations across individuals and species. In: Immunity, 2019, vol. 50, n° 5, p. 1317-1334.e10. doi: 10.1016/j.immuni.2019.03.009 https://archive-ouverte.unige.ch/unige:154045

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Deposited on : 2021-08-24

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