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Scientific article
Open access
English

Visualization of activated T cells by OX40-immunoPET as a strategy for diagnosis of acute Graft-versus-Host-Disease

Published inCancer Research, vol. 80, no. 21, p. 4780-4790
Publication date2020
Abstract

Graft versus host disease (GvHD) is a major complication of allogeneic hematopoietic cell transplantation (HCT), mediated primarily by donor T cells that become activated and attack host tissues. Non-invasive strategies detecting T cell activation would allow for early diagnosis and possibly more effective management of HCT recipients. Positron emission tomography (PET) imaging is a sensitive and clinically relevant modality ideal for GvHD diagnosis and there is a strong rationale for the use of PET tracers that can monitor T cell activation and expansion with high specificity. The tumor necrosis factor (TNF) receptor superfamily member OX40 (CD134) is a cell surface marker that is highly specific for activated T cells, is upregulated during GvHD, and mediates disease pathogenesis. We recently reported the development of an antibody-based activated T cell imaging agent targeting OX40. In the present study, we visualize the dynamics of OX40 expression in a major histocompatibility complex (MHC)-mismatch mouse model of acute GvHD using OX40-immunoPET. This approach enabled visualization of T cell activation at early stages of disease, prior to overt clinical symptoms with high sensitivity and specificity. This study highlights the potential utility of the OX40 PET imaging as a new strategy for GvHD diagnosis and therapy monitoring.

Keywords
  • Animals
  • Antibodies, Monoclonal/pharmacology
  • Copper Radioisotopes/pharmacology
  • Graft vs Host Disease/immunology
  • Lymphocyte Activation
  • Mice
  • Positron Emission Tomography Computed Tomography/methods
  • Radiopharmaceuticals/pharmacology
  • Receptors, OX40/analysis
  • Receptors, OX40/metabolism
  • T-Lymphocytes
  • Tissue Distribution
Affiliation Not a UNIGE publication
Funding
  • Autre - Swiss Cancer League BIL KLS 3806-02-201
Citation (ISO format)
ALAM, Israt S et al. Visualization of activated T cells by OX40-immunoPET as a strategy for diagnosis of acute Graft-versus-Host-Disease. In: Cancer Research, 2020, vol. 80, n° 21, p. 4780–4790. doi: 10.1158/0008-5472.CAN-20-1149
Main files (2)
Article (Published version)
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Article (Accepted version)
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Identifiers
ISSN of the journal0008-5472
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Technical informations

Creation10/28/2020 9:12:00 AM
First validation10/28/2020 9:12:00 AM
Update time03/16/2023 12:54:52 AM
Status update03/16/2023 12:54:51 AM
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