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Scientific article
English

Pseudomonads as Antagonists of Plant Pathogens in the Rhizosphere: Role of the Antibiotic 2,4-Diacetylphloroglucinol in the Suppression of Black Root Rot of Tobacco

Published inSymbiosis, vol. 9, p. 327-341
Publication date1990
Abstract

Pseudomonas fluorescens strain CHAO is an effective biocontrol agent of diseases caused by soilborne plant pathogens. Strain CHAO produces several secondary metabolites, notably cyanide, acetylpheoroglucinols and pyoluteorin. Cyanide plays an important role in the suppression of black root rot of tobacco, caused by Thielaviopsis basicola. A mutant, CHA625, has now been isolated, which does not produce 2,4-diacetylphloroglucinol. Strain CHA625 suppressed black root rot to a distinctly smaller extent than did wild-type CHAO under gnotobiotic conditions. A cosmid obtained from a genomic library of strain CHAO restored the ability of strain CHA625 to produce this metabolite and to suppress disease. Addition of synthetic 2,4-diacetylphloroglucinol to soil reduced disease severity in the absence of bacteria. These results suggest that the production of 2,4-diacetylphloroglucinol by P. fluorescens strain CHAO is another important factor, in addition to cyanide, in the suppression of black root rot of tobacco.

Keywords
  • Biocontrol
  • Soilborne pathogens
  • Pseudomonas
  • Antibiotics
  • Phloroglucinol
  • Cyanide
Funding
  • Swiss National Science Foundation - 3.100-25321
  • Swiss National Science Foundation - 3.133-085
Citation (ISO format)
KEEL, C. et al. Pseudomonads as Antagonists of Plant Pathogens in the Rhizosphere: Role of the Antibiotic 2,4-Diacetylphloroglucinol in the Suppression of Black Root Rot of Tobacco. In: Symbiosis, 1990, vol. 9, p. 327–341.
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Article (Published version)
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Identifiers
  • PID : unige:152909
ISSN of the journal0334-5114
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