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Book chapter
Open access
English

Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS

PublisherNew York, NY : Humana Press, Springer
Publication date2021
Abstract

Hydrophilic interaction chromatography (HILIC) coupled to mass spectrometry (MS) is considered as the reference analytical technique for glycans profiling, especially for the characterization of glycosylated protein therapeutics such as monoclonal antibodies (mAbs) and mAbs-related products. Although HILIC/MS is mainly known to profile enzymatically released and fluorescently labeled N-glycans, the recent commercialization of new widepore HILIC amide bonded stationary phases packed with sub-2 μm particles has allowed for remarkable separations also at the subunit level. Here, we describe a simple protocol to perform the mAb glycans profiling at subunit level by HILIC/MS.

Keywords
  • Hydrophilic interaction chromatography
  • Mass spectrometry
  • N-glycosylation
  • Biopharmaceutical proteins
  • Protocol
  • Monoclonal antibodies
Citation (ISO format)
D’ATRI, Valentina, GUILLARME, Davy. Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS. In: Mass Spectrometry of Glycoproteins. Methods in Molecular Biology. New York, NY : Humana Press, Springer, 2021. doi: 10.1007/978-1-0716-1241-5_6
Main files (1)
Book chapter (Published version)
accessLevelPublic
Identifiers
ISBN978-1-0716-1240-8
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Technical informations

Creation06/22/2021 5:00:00 PM
First validation06/22/2021 5:00:00 PM
Update time03/16/2023 12:48:01 AM
Status update03/16/2023 12:48:00 AM
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