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Scientific article
Open access
English

Cardiotrophin-1 Deficiency Abrogates Atherosclerosis Progression

Published inScientific Reports, vol. 10, no. 1, 5791
Publication date2020
Abstract

Cardiotrophin-1 (CT-1) is associated with cardiovascular (CV) diseases. We investigated the effect of CT-1 deficiency in the development and progression of atherosclerosis in double knockout Apoe-/-ct-1-/- mice. Apoe-/- C57Bl/6 or Apoe-/-ct-1-/- C57Bl/6 mice were fed a normal chow diet (NCD) or a high-cholesterol diet (HCD). After sacrifice, serum triglycerides, total cholesterol, low-density lipoprotein cholesterol (LDL-C), free fatty acids and systemic paracrine factors were measured. Intraplaque lipid and collagen content were quantified in the aortic sections. Immune cell populations in spleen, lymph nodes and aorta were analysis by flow cytometry. Apoe-/-ct-1-/- mice in accelerated atherosclerosis exhibited a reduction of total cholesterol, LDL-C, atherosclerotic plaques size in the aortic root and in the abdominal aorta and improved plaque stability in comparison to Apoe-/- mice. CT-1 deficiency in Apoe-/- mice on (HCD) promoted atheroprotective immune cell responses, as demonstrated by a rise in plasma anti-inflammatory immune cell populations (regulatory T cells, Tregs; regulatory B cells, Bregs and B1a cells) and atheroprotective IgM antibodies. CT-1 deficiency in advanced atherosclerosis mediated regulation of paracrine factors, such as interleukin (IL)-3, IL-6, IL-9, IL-15, IL-27, CXCL5, MCP-3, MIP-1α and MIP-1β. In a model of advanced atherosclerosis, CT-1 deficiency induced anti-inflammatory and atheroprotective effects which resulted in abrogation of atheroprogression.

Keywords
  • Animals
  • Aorta/pathology
  • Apolipoproteins E/genetics
  • Atherosclerosis/blood
  • Atherosclerosis/genetics
  • Atherosclerosis/pathology
  • Cytokines/genetics
  • Disease Models, Animal
  • Disease Progression
  • Gene Deletion
  • Male
  • Mice, Inbred C57BL
Citation (ISO format)
MITEVA, Kapka Todorova et al. Cardiotrophin-1 Deficiency Abrogates Atherosclerosis Progression. In: Scientific Reports, 2020, vol. 10, n° 1, p. 5791. doi: 10.1038/s41598-020-62596-6
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Identifiers
ISSN of the journal2045-2322
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Creation11/04/2020 11:52:00 AM
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Update time03/16/2023 12:47:44 AM
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