en
Scientific article
English

Murine gamma-herpesvirus 68 glycoprotein 150 protects against virus-induced mononucleosis: a model system for gamma-herpesvirus vaccination

Published inVaccine, vol. 17, no. 2, p. 152-157
Publication date1999
Abstract

Murine gamma-herpesvirus 68 (MHV-68) is a model for the study of the pathogenesis of gamma-herpesviruses. Epstein-Barr virus (EBV) is a highly related gamma-herpesvirus that causes significant disease in humans. The major membrane antigen gp350 of EBV is a candidate vaccine antigen for protection against EBV-related disease. An MHV-68 glycoprotein, gp150, has significant homology to EBV gp350. We have therefore used the MHV-68 gp150 to model the potential efficacy of EBV gp350 in protecting from virus-associated disease. A recombinant vaccinia virus expressing MHV-68 gp150 was constructed. This recombinant vaccinia virus was used to infect mice via the subcutaneous route. This vaccination resulted in production of MHV-68-neutralising antibodies. Mice were then challenged intra-nasally with MHV-68. MHV-68-associated mononucleosis was virtually abrogated in immunised mice. However, mice did establish MHV-68 latency. The results suggest that gp350 may be effective as an immunogen to prevent EBV-associated infectious mononucleosis in humans that are EBV-seronegative.

Keywords
  • Animals
  • Cell Line
  • Disease Models
  • Animal
  • Epstein-Barr Virus Infections/immunology/prevention & control
  • Female
  • Gammaherpesvirinae/immunology
  • Glycoproteins/immunology
  • Herpesviridae Infections/immunology/prevention & control
  • Herpesvirus 4
  • Human/immunology
  • Humans
  • Infectious Mononucleosis/immunology/prevention & control
  • Mice
  • Mice
  • Inbred BALB C
  • Spleen/cytology/immunology/virology
  • Vaccines
  • Synthetic/pharmacology
  • Viral Matrix Proteins/immunology
  • Viral Proteins/immunology
  • Viral Vaccines/pharmacology
Citation (ISO format)
STEWART, J P et al. Murine gamma-herpesvirus 68 glycoprotein 150 protects against virus-induced mononucleosis: a model system for gamma-herpesvirus vaccination. In: Vaccine, 1999, vol. 17, n° 2, p. 152–157. doi: 10.1016/s0264-410x(98)00190-x
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ISSN of the journal0264-410X
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