Scientific article
English

Murine gamma-herpesvirus 68 glycoprotein 150 protects against virus-induced mononucleosis: a model system for gamma-herpesvirus vaccination

Published inVaccine, vol. 17, no. 2, p. 152-157
Publication date1999
Abstract

Murine gamma-herpesvirus 68 (MHV-68) is a model for the study of the pathogenesis of gamma-herpesviruses. Epstein-Barr virus (EBV) is a highly related gamma-herpesvirus that causes significant disease in humans. The major membrane antigen gp350 of EBV is a candidate vaccine antigen for protection against EBV-related disease. An MHV-68 glycoprotein, gp150, has significant homology to EBV gp350. We have therefore used the MHV-68 gp150 to model the potential efficacy of EBV gp350 in protecting from virus-associated disease. A recombinant vaccinia virus expressing MHV-68 gp150 was constructed. This recombinant vaccinia virus was used to infect mice via the subcutaneous route. This vaccination resulted in production of MHV-68-neutralising antibodies. Mice were then challenged intra-nasally with MHV-68. MHV-68-associated mononucleosis was virtually abrogated in immunised mice. However, mice did establish MHV-68 latency. The results suggest that gp350 may be effective as an immunogen to prevent EBV-associated infectious mononucleosis in humans that are EBV-seronegative.

Keywords
  • Animals
  • Cell Line
  • Disease Models
  • Animal
  • Epstein-Barr Virus Infections/immunology/prevention & control
  • Female
  • Gammaherpesvirinae/immunology
  • Glycoproteins/immunology
  • Herpesviridae Infections/immunology/prevention & control
  • Herpesvirus 4
  • Human/immunology
  • Humans
  • Infectious Mononucleosis/immunology/prevention & control
  • Mice
  • Mice
  • Inbred BALB C
  • Spleen/cytology/immunology/virology
  • Vaccines
  • Synthetic/pharmacology
  • Viral Matrix Proteins/immunology
  • Viral Proteins/immunology
  • Viral Vaccines/pharmacology
Citation (ISO format)
STEWART, J P et al. Murine gamma-herpesvirus 68 glycoprotein 150 protects against virus-induced mononucleosis: a model system for gamma-herpesvirus vaccination. In: Vaccine, 1999, vol. 17, n° 2, p. 152–157. doi: 10.1016/s0264-410x(98)00190-x
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0264-410X
150views
0downloads

Technical informations

Creation10/22/2020 4:58:00 PM
First validation10/22/2020 4:58:00 PM
Update time03/16/2023 12:27:25 AM
Status update03/16/2023 12:27:24 AM
Last indexation10/31/2024 9:56:14 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack