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Scientific article
English

A Procedure for Northern Blot Analysis of Native RNA

Published inAnalytical Biochemistry, vol. 159, no. 1, p. 227-232
Publication date1986
Abstract

We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (GeneScreen) followed by uv irradiation. The sensitivity of the method using native RNA was found to be equivalent to that obtained with denatured RNA.

Keywords
  • RNA
  • mRNA
  • Gel electrophoresis of nucleic acids
  • Nucleic acid hybridization
  • Nylon membrane
  • Uv crosslinking
Funding
  • Swiss National Science Foundation - 3.567.83
  • Swiss National Science Foundation - 3.079.84
Citation (ISO format)
KHANDJIAN, Edouard W., MERIC, Claude. A Procedure for Northern Blot Analysis of Native RNA. In: Analytical Biochemistry, 1986, vol. 159, n° 1, p. 227–232. doi: 10.1016/0003-2697(86)90332-5
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Article (Published version)
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Identifiers
ISSN of the journal0003-2697
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