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Scientific article
English

Transfer RNA gene mapping studies on chloroplast DNA from Chlamydomonas reinhardii

Published inPlant Science, vol. 39, no. 2, p. 133-140
Publication date1985
Abstract

Total tRNA of Chlamydomonas reinhardii was fractionated by 2-dimensional gel electrophoresis. Sixteen tRNAs specific for eleven amino acids could be identified by aminoacylation with Escherichia coli tRNA synthetases. Hybridization of these tRNAs with chloroplast restriction fragments allowed for the localization of the genes of tRNATyr, tRNAPro, tRNAPhe (2 genes), tRNAIle (2 genes) and tRNAHis (2 genes) on the chloroplast genome of C. reinhardii. The genes for tRNAAla (2 genes), tRNAAsn and tRNALeu were mapped by using individual chloroplast tRNAs from higher plants as probes.

Keywords
  • tRNA
  • Chloroplast DNA
  • Chlamydomonas reinhardii
Funding
  • Swiss National Science Foundation - 3.258.082
  • Swiss National Science Foundation - 3.587.084
Citation (ISO format)
BERGMANN, Peter Willi et al. Transfer RNA gene mapping studies on chloroplast DNA from <i>Chlamydomonas reinhardii</i>. In: Plant Science, 1985, vol. 39, n° 2, p. 133–140. doi: 10.1016/0168-9452(85)90104-9
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Article (Published version)
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ISSN of the journal0168-9452
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