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Title

Nucleotide Sequences Responsible for the Thermal Inducibility of the Drosophila Small Heat-Shock Protein Genes in Monkey COS Cells

Authors
Ayme-Southgate, Agnes
Southgate, Richard
Published in Journal of Molecular Biology. 1985, vol. 182, no. 4, p. 469-475
Abstract The promoter regions of the Drosophila melanogaster small heat-shock protein genes have been analysed in order to localize those sequences responsible for their heat-shock transcriptional inducibility. Different lengths of the 5′ DNA sequences of these four genes were each fused individually to the Herpes simplex virus thymidine kinase (HSV-tk) transcription unit. These hybrid genes were constructed in a simian virus 40 recombinant vector for transfection in permissive monkey COS cells and tested for their heat-shock inducibility. The hsp22/HSV-tk and hsp26/HSV-tk fusion genes were found to be heat-inducible at 43 °C, giving rise to correctly initiated transcripts, but transcriptionally quiescent at 37 °C (control temperature). The hsp23 and hsp27 fusion gene constructs are, however, not heat-shock-inducible; no transcripts being detectable from hsp27/HSV-tk constructs at either temperature and all hsp23/HSV-tk clones being faithfully but constitutively expressed at low levels at both temperatures. By testing a series of 5′ deletion mutants in hsp22/HSV-tk, a homologous sequence located adjacent to the TATA box in both the hsp22 and hsp26 genes was identified as being responsible for their heat-shock activation. This control element corresponds to the Pelham “consensus sequence”, previously described for the Drosophila hsp70 genes. The possible modes of transcriptional induction of all four genes are discussed.
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Swiss National Science Foundation: 3.512.79
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AYME-SOUTHGATE, Agnes, SOUTHGATE, Richard, TISSIERES, Alfred. Nucleotide Sequences Responsible for the Thermal Inducibility of the Drosophila Small Heat-Shock Protein Genes in Monkey COS Cells. In: Journal of Molecular Biology, 1985, vol. 182, n° 4, p. 469-475. doi: 10.1016/0022-2836(85)90233-5 https://archive-ouverte.unige.ch/unige:150763

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