Scientific article

Hybrid plasmids carrying part of the Rous sarcoma virus-specific leader sequence

Publication date1981

‘Strong-stop' DNA, complementary to the 5′-terminal 101 nucleotides of Rous sarcoma virus genomic RNA and subgenomic mRNAs can be used to isolate RSV-specific mRNA from infected cells. To analyse leader sequences of these mRNAs and to study the mechanism of their translation, relatively large quantities of strong-stop DNA are required. The construction of four plasmids carrying useful strong-stop DNA sequences for mRNA isolation is described. One such hybrid plasmid carries a DNA insertion containing sequences derived from a region starting 42 nucleotides from the 5′-end of RSV RNA, and ending at the tRNATry primer attachment site. Since this plasmid lacks the 20-nucleotide terminal repeat sequence it can be used for the specific isolation of 5′-end fragments of RSV-specific mRNAs and of complementary DNA transcripts of these fragments.

  • 'Strong-stop DNA'
  • mRNA
  • Leader sequence
  • Plasmid
  • Translation
  • Rous sarcoma virus
  • Swiss National Science Foundation - 3.310.78
  • Swiss National Science Foundation - 3.512.79
Citation (ISO format)
BROMLEY, Peter A., VOELLMY, Richard, SPAHR, Pierre-François. Hybrid plasmids carrying part of the Rous sarcoma virus-specific leader sequence. In: Biochimica et Biophysica Acta - Nucleic Acids and Protein Synthesis, 1981, vol. 655, n° 1, p. 41–48. doi: 10.1016/0005-2787(81)90064-2
Main files (1)
Article (Published version)
ISSN of the journal0005-2787

Technical informations

Creation03/18/2021 8:31:00 AM
First validation03/18/2021 8:31:00 AM
Update time03/16/2023 12:16:48 AM
Status update03/16/2023 12:16:48 AM
Last indexation10/19/2023 2:59:44 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack