Article (Published version) (1.5 MB) - 
Limited access to UNIGEOther version: https://linkinghub.elsevier.com/retrieve/pii/037811198190161X
Highlights
Scientific Article
unige:150493 
 |
The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication |
|
| Authors | ||
| Published in | Gene. 1981, vol. 14, no. 4, p. 289-299 | |
| Abstract | The plasmid pBR325 is a cloning vector constructed in vitro by addition of the chloramphenicol resistance (Cmr) gene of an IS1-flanked transposon to pBR322 (Bolivar, 1978). It is a 5995 bp plasmid carruing to sequence originating from ISl. DNA-sequence data suggest that its Cmr segment was derived from a Cm transposon longer than Tn9. The plasmid pBR325 carries between the Cmr and the Tcr genes a 482 bp sequence which duplicates, in the opposite orientation, a section of pBR322 located at the end of the Tcr gene. The same structure was found in pBR328, a deletion derivative of pBR325 (Soberon et al., 1980). The possible implications of this inverted duplication on cloning experiments are discussed. | |
| Keywords | DNA sequence pBR325 — Snap-back structure — Restriction map — Chloromaphenicolresistance genes — IS1 element — Transposons | |
| Identifiers | ||
| Full text |
Article (Published version) (1.5 MB) - Limited access to UNIGE Other version: https://linkinghub.elsevier.com/retrieve/pii/037811198190161X |
|
| Structures | ||
| Projects | FNS: 3.479.79 FNS: 3.591.79 | |
| Citation (ISO format) | PRENTKI, Pierre et al. The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication. In: Gene, 1981, vol. 14, n° 4, p. 289-299. doi: 10.1016/0378-1119(81)90161-X https://archive-ouverte.unige.ch/unige:150493 |
53 hits 
0 download 
Contact an author
Update