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Scientific article
English

The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication

Published inGene, vol. 14, no. 4, p. 289-299
Publication date1981
Abstract

The plasmid pBR325 is a cloning vector constructed in vitro by addition of the chloramphenicol resistance (Cmr) gene of an IS1-flanked transposon to pBR322 (Bolivar, 1978). It is a 5995 bp plasmid carruing to sequence originating from ISl. DNA-sequence data suggest that its Cmr segment was derived from a Cm transposon longer than Tn9. The plasmid pBR325 carries between the Cmr and the Tcr genes a 482 bp sequence which duplicates, in the opposite orientation, a section of pBR322 located at the end of the Tcr gene. The same structure was found in pBR328, a deletion derivative of pBR325 (Soberon et al., 1980). The possible implications of this inverted duplication on cloning experiments are discussed.

Keywords
  • DNA sequence pBR325
  • Snap-back structure
  • Restriction map
  • Chloromaphenicolresistance genes
  • IS1 element
  • Transposons
Funding
  • Swiss National Science Foundation - 3.479.79
  • Swiss National Science Foundation - 3.591.79
Citation (ISO format)
PRENTKI, Pierre et al. The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication. In: Gene, 1981, vol. 14, n° 4, p. 289–299. doi: 10.1016/0378-1119(81)90161-X
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Identifiers
ISSN of the journal0378-1119
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