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The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication

Iida, Shigeru
Meyer, Jürg
Published in Gene. 1981, vol. 14, no. 4, p. 289-299
Abstract The plasmid pBR325 is a cloning vector constructed in vitro by addition of the chloramphenicol resistance (Cmr) gene of an IS1-flanked transposon to pBR322 (Bolivar, 1978). It is a 5995 bp plasmid carruing to sequence originating from ISl. DNA-sequence data suggest that its Cmr segment was derived from a Cm transposon longer than Tn9. The plasmid pBR325 carries between the Cmr and the Tcr genes a 482 bp sequence which duplicates, in the opposite orientation, a section of pBR322 located at the end of the Tcr gene. The same structure was found in pBR328, a deletion derivative of pBR325 (Soberon et al., 1980). The possible implications of this inverted duplication on cloning experiments are discussed.
Keywords DNA sequence pBR325Snap-back structureRestriction mapChloromaphenicolresistance genesIS1 elementTransposons
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Swiss National Science Foundation: 3.479.79
Swiss National Science Foundation: 3.591.79
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PRENTKI, Pierre et al. The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication. In: Gene, 1981, vol. 14, n° 4, p. 289-299. doi: 10.1016/0378-1119(81)90161-X https://archive-ouverte.unige.ch/unige:150493

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