en
Scientific article
Open access
English

Evaluation of a nanoflow interface based on the triple-tube coaxial sheath-flow sprayer for capillary electrophoresis-mass spectrometry coupling in metabolomics

Published inJournal of Chromatography. A, vol. 1641, 461982
Publication date2021
Abstract

The performance of an original CE-MS interface that allows the in-axis positioning of the electrospray with respect to the MS inlet was evaluated. The variations in the geometrical alignment of this configu- ration in the absence of a nebulizing gas afforded a significant reduction in the sheath-liquid flow rate from 3 μL/min to as low as 300 nL/min. The sheath liquid and BGE were respectively composed of H2O—iPrOH–CH3COOH 50:50:1 (v/v/v) and 10% acetic acid (pH 2.2). A significant gain in sensitivity was ob- tained, and it was correlated to the effective mobility of the analytes. Compounds with low mobility val- ues showed a greater sensitivity gain. Special attention was paid to the detection of proteinogenic amino acids. Linear response functions were obtained from 15 ng/mL to 500 ng/mL. The limits of quantification, as low as 34.3 ng/mL, were improved by a factor of up to six compared to the conventional configu- ration. The in-axis setup was ultimately applied to the absolute quantification of four important amino acids, alanine, tyrosine, methionine and valine, in standard reference material (NIST plasma). The accura- cies ranged from 78 to 113%, thus demonstrating the potential of this configuration for metabolomics.

Keywords
  • Capillary electrophoresis
  • CE-ESI-MS
  • Mass spectrometry
  • Nanoflow interface
  • Amino acids
Citation (ISO format)
FERRE, Sabrina et al. Evaluation of a nanoflow interface based on the triple-tube coaxial sheath-flow sprayer for capillary electrophoresis-mass spectrometry coupling in metabolomics. In: Journal of Chromatography. A, 2021, vol. 1641, p. 461982. doi: 10.1016/j.chroma.2021.461982
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal0021-9673
251views
126downloads

Technical informations

Creation03/09/2021 8:24:00 AM
First validation03/09/2021 8:24:00 AM
Update time03/16/2023 12:14:22 AM
Status update03/16/2023 12:14:22 AM
Last indexation05/06/2024 6:47:43 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack