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Evaluation of a nanoflow interface based on the triple-tube coaxial sheath-flow sprayer for capillary electrophoresis-mass spectrometry coupling in metabolomics

Contributors

Ferre, Sabrina; Drouin, Nicolas; Gonzalez Ruiz, Victor; Rudaz, Serge

Published in

Journal of Chromatography. A, vol. 1641, 461982

Publication date

2021

Abstract

The performance of an original CE-MS interface that allows the in-axis positioning of the electrospray with respect to the MS inlet was evaluated. The variations in the geometrical alignment of this configu- ration in the absence of a nebulizing gas afforded a significant reduction in the sheath-liquid flow rate from 3 μL/min to as low as 300 nL/min. The sheath liquid and BGE were respectively composed of H2O—iPrOH–CH3COOH 50:50:1 (v/v/v) and 10% acetic acid (pH 2.2). A significant gain in sensitivity was ob- tained, and it was correlated to the effective mobility of the analytes. Compounds with low mobility val- ues showed a greater sensitivity gain. Special attention was paid to the detection of proteinogenic amino acids. Linear response functions were obtained from 15 ng/mL to 500 ng/mL. The limits of quantification, as low as 34.3 ng/mL, were improved by a factor of up to six compared to the conventional configu- ration. The in-axis setup was ultimately applied to the absolute quantification of four important amino acids, alanine, tyrosine, methionine and valine, in standard reference material (NIST plasma). The accura- cies ranged from 78 to 113%, thus demonstrating the potential of this configuration for metabolomics.

Keywords

  • Capillary electrophoresis
  • CE-ESI-MS
  • Mass spectrometry
  • Nanoflow interface
  • Amino acids

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

FERRE, Sabrina et al. Evaluation of a nanoflow interface based on the triple-tube coaxial sheath-flow sprayer for capillary electrophoresis-mass spectrometry coupling in metabolomics. In: Journal of Chromatography. A, 2021, vol. 1641, p. 461982. doi: 10.1016/j.chroma.2021.461982

Main files (1)

Article (Published version)
CC BY

Identifiers

Additional URL for this publication

https://linkinghub.elsevier.com/retrieve/pii/S0021967321001060

Journal ISSN

0021-9673
333views
443downloads

Technical informations

Creation

09/03/2021 08:24:00

First validation

09/03/2021 08:24:00

Update

16/03/2023 00:14:22

Status update

16/03/2023 00:14:22

Last indexation

18/03/2025 09:25:16
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