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Massive Heat-Shock Polypeptide Synthesis in Late Chicken Embryos: Convenient System for Study of Protein Synthesis in Highly Differentiated Organisms

Published inMolecular and Cellular Biology, vol. 2, no. 5, p. 479-483
Publication date1982
Abstract

In cultured eucaryotic cells, heat treatments specifically induced the rapid synthesis of the so-called heat-shock polypeptides. To ascertain the physiological importance of this phenomenon for highly differentiated organisms, we attempted to determine whether the heat-shock response occurs in a living endothermic organism at extreme temperatures, and if so, whether the response is organ specific. We developed a procedure to label proteins efficiently in 5- to 18-day-old chicken embryos. Heat-shock polypeptides of identical sizes of 85,000, 70,000, and 25,000 daltons were synthesized predominantly in chicken embryo fibroblasts and in many different organs of 18-day-old embryos at 42.5 to 44 degrees C.

Funding
  • Swiss National Science Foundation - 3.664.80
  • Swiss National Science Foundation - 3.512.79
Citation (ISO format)
VOELLMY, Richard, BROMLEY, Peter A. Massive Heat-Shock Polypeptide Synthesis in Late Chicken Embryos: Convenient System for Study of Protein Synthesis in Highly Differentiated Organisms. In: Molecular and Cellular Biology, 1982, vol. 2, n° 5, p. 479–483. doi: 10.1128/MCB.2.5.479
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ISSN of the journal0270-7306
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