Scientific article
Open access

An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission

Published inCell, vol. 182, no. 5, p. 1140-1155
Publication date2020

The endosomal sorting complex required for transport-III (ESCRT-III) catalyzes membrane fission from within membrane necks, a process that is essential for many cellular functions, from cell division to lysosome degradation and autophagy. How it breaks membranes, though, remains unknown. Here, we characterize a sequential polymerization of ESCRT-III subunits that, driven by a recruitment cascade and by continuous subunit-turnover powered by the ATPase Vps4, induces membrane deformation and fission. During this process, the exchange of Vps24 for Did2 induces a tilt in the polymer-membrane interface, which triggers transition from flat spiral polymers to helical filament to drive the formation of membrane protrusions, and ends with the formation of a highly constricted Did2-Ist1 co-polymer that we show is competent to promote fission when bound on the inside of membrane necks. Overall, our results suggest a mechanism of stepwise changes in ESCRT-III filament structure and mechanical properties via exchange of the filament subunits to catalyze ESCRT-III activity.

  • CHMP1
  • CHMP4
  • Did2
  • Ist1
  • Snf7
  • Vps2
  • In vitro reconstitution
  • Membrane fission
  • Membrane remodeling
Research group
Citation (ISO format)
PFITZNER, Anna-Katharina et al. An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission. In: Cell, 2020, vol. 182, n° 5, p. 1140–1155. doi: 10.1016/j.cell.2020.07.021
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Article (Published version)
ISSN of the journal0092-8674

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