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Increased Expression of the dnaA Gene Has No Effect on DNA Replication in a dnaA+ Strain of Escherichia coli

Published inMolecular and General Genetics, vol. 192, no. 3, p. 506-508
Publication date1983
Abstract

We have constructed a pBR322 plasmid derivative which expresses dnaA protein under the control of the E. coli lac UV5 promotor. Expression of the dnaA protein from the plasmid is inducible by isopropyl-β-D-thiogalactoside. In a dnaA⁺ strain induction has no effect on the accumulation of DNA. In contrast, in a thermosensitive dnaA46 strain, induction, at either the permissive or the nonpermissive temperature, results in an immediate stimulation of DNA accumulation. We conclude that, while in a dnaA46 strain dnaA protein limits DNA replication, in a dnaA⁺ strain dnaA protein activity does not control the timing of replication initiation.

Keywords
  • Protein activity
  • dnaA Gene
  • Replication initiation
  • Nonpermissive temperature
  • pBR322 Plasmid
Funding
  • Swiss National Science Foundation - 3.169.0.81
  • Autre - NIH GM25948
Citation (ISO format)
CHURCHWARD, Gordon, HOLMANS, P., BREMER, Hans. Increased Expression of the <i>dnaA</i> Gene Has No Effect on DNA Replication in a <i>dnaA</i><sup>+</sup> Strain of <i>Escherichia coli</i>. In: Molecular and General Genetics, 1983, vol. 192, n° 3, p. 506–508. doi: 10.1007/BF00392197
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Article (Published version)
Identifiers
ISSN of the journal0026-8925
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