en
Scientific article
English

First DNA Sequence of a Chloroplast Mutation: A Missense Alteration in the Ribulosebisphosphate Carboxylase Large Subunit Gene

Published inPlasmid, vol. 9, no. 3, p. 321-324
Publication date1983
Abstract

Sequence comparison of the chloroplast genes of the large subunit of ribulosebisphosphate carboxylase from wild-type and from a uniparental mutant of the green unicellular algaChlamydomonas reinhardii has revealed a single nucleotide change. The corresponding Gly to Asp amino acid substitution would introduce a negative charge into the presumptive substrate binding region of the enzyme and would explain the inactivity of the mutant protein. This is the first chloroplast mutation whose DNA sequence is known. Our results establish the first exact point of correlation between the physical map of the chloroplast genome ofC. reinhardii and a specific genetic locus.

Funding
  • Swiss National Science Foundation - 3.659.0.80
Citation (ISO format)
DRON, Michel Philippe et al. First DNA Sequence of a Chloroplast Mutation: A Missense Alteration in the Ribulosebisphosphate Carboxylase Large Subunit Gene. In: Plasmid, 1983, vol. 9, n° 3, p. 321–324. doi: 10.1016/0147-619X(83)90009-4
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0147-619X
159views
0downloads

Technical informations

Creation02/24/2021 12:24:00 PM
First validation02/24/2021 12:24:00 PM
Update time03/16/2023 12:10:09 AM
Status update03/16/2023 12:10:09 AM
Last indexation01/17/2024 12:36:59 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack