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Scientific article
English

First DNA Sequence of a Chloroplast Mutation: A Missense Alteration in the Ribulosebisphosphate Carboxylase Large Subunit Gene

Published inPlasmid, vol. 9, no. 3, p. 321-324
Publication date1983
Abstract

Sequence comparison of the chloroplast genes of the large subunit of ribulosebisphosphate carboxylase from wild-type and from a uniparental mutant of the green unicellular algaChlamydomonas reinhardii has revealed a single nucleotide change. The corresponding Gly to Asp amino acid substitution would introduce a negative charge into the presumptive substrate binding region of the enzyme and would explain the inactivity of the mutant protein. This is the first chloroplast mutation whose DNA sequence is known. Our results establish the first exact point of correlation between the physical map of the chloroplast genome ofC. reinhardii and a specific genetic locus.

Funding
  • Swiss National Science Foundation - 3.659.0.80
Citation (ISO format)
DRON, Michel Philippe et al. First DNA Sequence of a Chloroplast Mutation: A Missense Alteration in the Ribulosebisphosphate Carboxylase Large Subunit Gene. In: Plasmid, 1983, vol. 9, n° 3, p. 321–324. doi: 10.1016/0147-619X(83)90009-4
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ISSN of the journal0147-619X
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