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Scientific article
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Association of Three Chicken Proteins with the 34 kD Target of Rous Sarcoma Virus Tyrosine Kinase

Published inExperimental Cell Research, vol. 169, no. 2, p. 419-431
Publication date1987
Abstract

Transformation of chicken embryo fibroblasts by avian retroviruses induces the tyrosine phosphorylation of a 34–39 kD cellular protein (p34). In vitro, p34 isolated from intestine interacts with F-actin in a Ca²⁺-dependent manner. We report here that, in the absence of Ca²⁺ chelators, three proteins co-purified with p34 extracted from a cytosolic or membrane fraction of chicken embryo fibroblasts; these two fractions account respectively for 10–20% and 50% of the total cellular p34. Isolated from the cytosoluble fraction of fibroblasts by sucrose gradient centrifugation and hydrophobic chromatography, p34 and the other proteins behaved as a homogeneous species upon non-denaturing gel electrophoresis, gel filtration, and CsCl density gradient centrifugation, thus indicating a strong association. Moreover, an analysis by electron microscopy following uranyl acetate staining revealed particles with a raspberry-like shape. This association was always disrupted by the calcium-chelating agent, EGTA.

Funding
  • Swiss National Science Foundation - 3.079.084
Citation (ISO format)
SIMON, Michel, ARRIGO, André-Patrick, SPAHR, Pierre-François. Association of Three Chicken Proteins with the 34 kD Target of Rous Sarcoma Virus Tyrosine Kinase. In: Experimental Cell Research, 1987, vol. 169, n° 2, p. 419–431. doi: 10.1016/0014-4827(87)90202-3
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ISSN of the journal0014-4827
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