en
Scientific article
English

Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii

Published inEMBO Journal, vol. 6, no. 2, p. 313-318
Publication date1987
Abstract

In Chlamydomonas reinhardtii the oxygen evolving enhancer protein 1 (OEE1), which is part of the oxygen evolving complex of photosystem II (PS II), is coded for by a single nuclear gene (psb1). The nuclear mutant FuD44 specifically lacks the OEE1 polypeptide and is completely deficient in photosynthetic oxygen evolution. In this mutant a 5 kb DNA insertion into the 5′ region of the psb1 gene results in the complete absence of OEE1 mRNA and protein. A revertant, FuD44‐R 2, which is capable of 30% of the photosynthetic oxygen evolution of wild‐type cells, has lost 4 kb of the 5 kb DNA insert, and accumulates both OEE1 mRNA and protein, although at levels somewhat less than those of wild‐type cells. Absence of the OEE1 protein in the FuD44 mutant does not affect the accumulation of other nuclear encoded PS II peripheral polypeptides. OEE1 absence does, however, result in a more rapid turnover of the chloroplast encoded PS II core polypeptides, thus resulting in a substantial deficiency of PS II core polypeptides in FuD44 cells. These PS II core proteins again accumulate in revertant FuD44‐R2 cells.

Keywords
  • Chlamydomonas reinhardtii
  • Photosystem II particles
  • Stability
  • Oxygen evolution
  • OEE1 protein requirement
Funding
  • Swiss National Science Foundation - 3.587-0.84
Citation (ISO format)
MAYFIELD, Stephen Patrick, BENNOUN, Pierre, ROCHAIX, Jean-David. Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in <i>Chlamydomonas reinhardtii</i>. In: EMBO Journal, 1987, vol. 6, n° 2, p. 313–318. doi: 10.1002/j.1460-2075.1987.tb04756.x
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0261-4189
117views
0downloads

Technical informations

Creation02/16/2021 3:03:00 PM
First validation02/16/2021 3:03:00 PM
Update time03/16/2023 12:06:26 AM
Status update03/16/2023 12:06:26 AM
Last indexation01/17/2024 12:30:21 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack