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SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism

Kress, Michel
Published in Oncogene. 1989, vol. 4, no. 5, p. 601-608
Abstract SV40 and polyoma virus induce a mitotic host reaction in confluent, Go-arrested primary mouse kidney cell cultures. To define the primary effects of infection we constructed a cDNA library corresponding to polyA+ mRNA isolated shortly after onset of polyoma T-antigen synthesis. By differential screening of the library we have isolated and then sequenced cDNA recombinant 24p3; determined by Northern blotting, 24p3 mRNA steady state levels increased in parallel with polyoma and SV40 T-antigen synthesis. Since this rapid and early increase was particularly striking (14-20 fold) in SV40-infected cells, we studied the molecular mechanism of induction in this virus-cell system. We show that wt SV40 large T-antigen is required for the increase in 24p3 mRNA levels. The results tend to exclude that this increase is due to an SV40-induced stabilization of the 24p3 mRNA, or to an SV40-induced stimulation of transcription of the 24p3 gene; they are compatible with the working hypothesis that SV40 large T-antigen increases the efficiency of processing, possibly splicing, of the 24p3 pre-mRNA. The biological implications of these results are discussed.
Keywords Amino acid sequenceAnimalsPolyomavirus transforming antigens/biosynthesisBase sequenceNorthern blottingCultured cellsGene expression regulationMiceMolecular sequence dataPolyomavirus/physiologyPost-transcriptional RNA processingMessenger RNA/metabolismSimian virus 40/genetics/physiologyGenetic transcription
PMID: 2542864
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HRABA-RENEVEY, Suzanne et al. SV40-induced expression of mouse gene 24p3 involves a post-transcriptional mechanism. In: Oncogene, 1989, vol. 4, n° 5, p. 601-608. https://archive-ouverte.unige.ch/unige:149338

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