Scientific article
Open access

A role for cytosolic isocitrate dehydrogenase as a negative regulator of glucose signaling for insulin secretion in pancreatic ß-cells

Published inPLOS ONE, vol. 8, no. 10, e77097
Publication date2013

Cytosolic NADPH may act as one of the signals that couple glucose metabolism to insulin secretion in the pancreatic ß-cell. NADPH levels in the cytoplasm are largely controlled by the cytosolic isoforms of malic enzyme and isocitrate dehydrogenase (IDHc). Some studies have provided evidence for a role of malic enzyme in glucose-induced insulin secretion (GIIS) via pyruvate cycling, but the role of IDHc in ß-cell signaling is unsettled. IDHc is an established component of the isocitrate/α-ketoglutarate shuttle that transfers reducing equivalents (NADPH) from the mitochondrion to the cytosol. This shuttle is energy consuming since it is coupled to nicotinamide nucleotide transhydrogenase that uses the mitochondrial proton gradient to produce mitochondrial NADPH and NAD(+) from NADP(+) and NADH. To determine whether flux through IDHc is positively or negatively linked to GIIS, we performed RNAi knockdown experiments in ß-cells. Reduced IDHc expression in INS 832/13 cells and isolated rat islet ß-cells resulted in enhanced GIIS. This effect was mediated at least in part via the KATP-independent amplification arm of GIIS. IDHc knockdown in INS 832/13 cells did not alter glucose oxidation but it reduced fatty acid oxidation and increased lipogenesis from glucose. Metabolome profiling in INS 832/13 cells showed that IDHc knockdown increased isocitrate and NADP(+) levels. It also increased the cellular contents of several metabolites linked to GIIS, in particular some Krebs cycle intermediates, acetyl-CoA, glutamate, cAMP and ATP. The results identify IDHc as a component of the emerging pathways that negatively regulate GIIS.

  • Animals
  • Cell Line, Tumor
  • Cytosol/enzymology
  • Energy Metabolism
  • Fatty Acids/metabolism
  • Gene Expression Regulation, Enzymologic/genetics
  • Gene Knockdown Techniques
  • Glucose/metabolism
  • Insulin/metabolism
  • Insulin Secretion
  • Insulin-Secreting Cells/cytology/enzymology/metabolism
  • Isocitrate Dehydrogenase/deficiency/genetics/metabolism
  • RNA, Small Interfering/genetics
  • Rats
  • Signal Transduction
Affiliation Not a UNIGE publication
Citation (ISO format)
GUAY, Claudiane et al. A role for cytosolic isocitrate dehydrogenase as a negative regulator of glucose signaling for insulin secretion in pancreatic ß-cells. In: PLOS ONE, 2013, vol. 8, n° 10, p. e77097. doi: 10.1371/journal.pone.0077097
Main files (1)
Article (Published version)
ISSN of the journal1932-6203

Technical informations

Creation01/14/2021 3:29:00 PM
First validation01/14/2021 3:29:00 PM
Update time03/16/2023 12:02:28 AM
Status update03/16/2023 12:02:27 AM
Last indexation02/12/2024 12:01:44 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack