Scientific article

Injectable rhBMP-2-loaded chitosan hydrogel composite: osteoinduction at ectopic site and in segmental long bone defect

Published inJournal of biomedical materials research. Part A, vol. 96, no. 1, p. 66-74
Publication date2011

Carriers for bone morphogenetic protein-2 (BMP-2) used in clinical practice still suffer from limitations such as insufficient protein retention. In addition, there is a clinical need for injectable carriers. The main objective of this study was to assess bone forming ability of rhBMP-2 combined either with chitosan hydrogel (rhBMP-2/CH) or chitosan hydrogel containing β-tricalcium phosphate (β-TCP) (rhBMP-2/CH/TCP). Formulations were first compared in a rat ectopic intramuscular bone formation model, and the optimal formulation was further evaluated in healing of 15-mm critical size defect in the radius of a rabbit. Three weeks after injection ectopically formed bone was analyzed by microcomputerized tomography (micro-CT) and histology. Significantly higher (4.7-fold) mineralized bone formation was observed in the rhBMP-2/CH/TCP group compared to rhBMP-2/CH group. In a pilot study, defect in a rabbit radius treated with rhBMP-2/CH/TCP showed incomplete regeneration at 8 weeks with composite leakage from the defect, indicating the need for formulation refinement when segmental defect repair is foreseen.

  • Animals
  • Biocompatible Materials/chemistry
  • Bone Morphogenetic Protein 2/chemistry/pharmacology
  • Bone and Bones/drug effects/pathology/physiology
  • Calcium Phosphates/chemistry
  • Chitosan/chemistry
  • Drug Carriers/chemistry
  • Hydrogels/chemistry
  • Injections
  • Male
  • Materials Testing
  • Osteogenesis/drug effects
  • Rabbits
  • Rats
  • Rats, Sprague-Dawley
  • X-Ray Microtomography
Citation (ISO format)
LUCA, Ludmila et al. Injectable rhBMP-2-loaded chitosan hydrogel composite: osteoinduction at ectopic site and in segmental long bone defect. In: Journal of biomedical materials research. Part A, 2011, vol. 96, n° 1, p. 66–74. doi: 10.1002/jbm.a.32957
Main files (1)
Article (Published version)
ISSN of the journal1549-3296

Technical informations

Creation03/29/2011 7:00:00 PM
First validation03/29/2011 7:00:00 PM
Update time03/14/2023 4:14:52 PM
Status update03/14/2023 4:14:52 PM
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