Preparation of Chlamydomonas Chloroplasts for the <i>in Vitro</i> Import of Polypeptide Precursors

Goldschmidt-Clermont, Michel P.; Malnoe, Pia Christina; Rochaix, Jean-David

doi:10.1104/pp.89.1.15

Scientific article

English

Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors

ContributorsGoldschmidt-Clermont, Michel P.; Malnoe, Pia Christina; Rochaix, Jean-David

Published inPlant Physiology, vol. 89, no. 1, p. 15-18

Publication date1989

Abstract

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.

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Citation (ISO format)

GOLDSCHMIDT-CLERMONT, Michel P., MALNOE, Pia Christina, ROCHAIX, Jean-David. Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors. In: Plant Physiology, 1989, vol. 89, n° 1, p. 15–18. doi: 10.1104/pp.89.1.15

Article (Published version)

Identifiers

PID : unige:148886
DOI : 10.1104/pp.89.1.15
PMID : 16666506

Journal ISSN0032-0889

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Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors

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