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Scientific article
English

Preparation of Chlamydomonas Chloroplasts for the in Vitro Import of Polypeptide Precursors

Published inPlant Physiology, vol. 89, no. 1, p. 15-18
Publication date1989
Abstract

To study the import of polypeptide precursors we have adapted and compared two procedures for the isolation of competent chloroplasts from the green unicellular alga, Chlamydomonas reinhardtii: silicasol gradient centrifugation and elutriation. The chloroplasts actively import the precursor of the small subunit of ribulose bisphosphate carboxylase-oxygenase in vitro.

Citation (ISO format)
GOLDSCHMIDT-CLERMONT, Michel P., MALNOE, Pia Christina, ROCHAIX, Jean-David. Preparation of Chlamydomonas Chloroplasts for the <i>in Vitro</i> Import of Polypeptide Precursors. In: Plant Physiology, 1989, vol. 89, n° 1, p. 15–18. doi: 10.1104/pp.89.1.15
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ISSN of the journal0032-0889
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